Akerlund T, Bernander R, Nordström K
Department of Microbiology, Uppsala University, Sweden.
Mol Microbiol. 1992 Aug;6(15):2073-83. doi: 10.1111/j.1365-2958.1992.tb01380.x.
In Escherichia coli minB mutants, cell division can take place at the cell poles as well as non-polarly in the cell. We have examined growth, division patterns, and nucleoid distribution in individual cells of a minC point mutant and a minB deletion mutant, and compared them to the corresponding wild-type strain and an intR1 strain in which the chromosome is over-replicated. The main findings were as follows. In the minB mutants, polar and non-polar divisions appeared to occur independently of each other. Furthermore, the timing of cell division in the cell cycle was found to be severely affected. In addition, nucleoid conformation and distribution were considerably disturbed. The results obtained call for a re-evaluation of the role of the MinB system in the E. coli cell cycle, and of the concept that limiting quanta of cell division factors are regularly produced during the cell cycle.
在大肠杆菌minB突变体中，细胞分裂既可以在细胞两极发生，也可以在细胞内非两极位置发生。我们检测了一个minC点突变体和一个minB缺失突变体单个细胞中的生长、分裂模式及类核分布，并将它们与相应的野生型菌株以及染色体过度复制的intR1菌株进行了比较。主要发现如下。在minB突变体中，两极和非两极分裂似乎相互独立发生。此外，发现细胞周期中细胞分裂的时间受到严重影响。另外，类核构象和分布也受到相当大的干扰。所获得的结果要求重新评估MinB系统在大肠杆菌细胞周期中的作用，以及细胞周期中定期产生有限数量细胞分裂因子这一概念。
