Critchfield J W, Keen C L
Department of Nutrition, University of California, Davis 95616.
Metabolism. 1992 Oct;41(10):1087-92. doi: 10.1016/0026-0495(92)90290-q.
Plasma from fasted adult male subjects was labeled in vitro with 54MnCl2 and then fractionated using several techniques. Molecular sieve chromatography showed that the major 54Mn-containing peak had a very low molecular weight (VLMW), although four other significant peaks, one of which corresponded to the mass of transferrin (Tf), were also observed. The 54Mn content of the Tf peak increased with increasing incubation time in vitro, suggesting the oxidation of Mn+2 to Mn+3 before its association with Tf. This time-dependent effect was verified using affinity chromatography consisting of immobilized anti-Tf. Electrophoretic analyses of plasma yielded equivocal results, indicating a limited value of this method for investigating plasma manganese localization. The above findings are discussed in the context of factors that influence the oxidation and metabolism of Mn2+ in human plasma.
来自禁食成年男性受试者的血浆在体外用54MnCl2进行标记，然后使用多种技术进行分离。分子筛色谱显示，主要的含54Mn峰具有非常低的分子量（VLMW），不过还观察到其他四个显著峰，其中一个对应转铁蛋白（Tf）的质量。Tf峰的54Mn含量随着体外孵育时间的增加而增加，表明在Mn+2与Tf结合之前其被氧化为Mn+3。使用固定化抗Tf的亲和色谱验证了这种时间依赖性效应。血浆的电泳分析结果不明确，表明该方法在研究血浆锰定位方面价值有限。在影响人血浆中Mn2+氧化和代谢的因素背景下讨论了上述发现。
