Tate G, Ishizawa M
Department of Pathology, Asahikawa Medical College, Hokkaido, Japan.
Microbiol Immunol. 1992;36(7):737-44. doi: 10.1111/j.1348-0421.1992.tb02076.x.
Genomic DNA obtained from a B lymphoblastoid cell line was digested with appropriate restriction endonuclease and hybridized with several probes specific for genes encoding HLA-DQ. Southern hybridization with a DQA1 3'untranslated (UT) region probe showed DQ2-type hybridization pattern in DR7DQ3 haplotype. On the contrary, DQB1 3'UT probe showed DQ3-type pattern in the same haplotype. Gene cloning and DNA sequencing analysis revealed a repetitive sequence, (TG)19, between DQA1 and DQB1 gene in the DR7DQ3 haplotype. These results suggest that a recombination event has occurred near this potential Z-DNA structure in the haplotype, DR7DQ3. The 3'UT region probes of DQA1 and DQB1 genes failed to detect restriction fragment length polymorphism (RFLP) differences between DR4DQ3 and DR4DQ4 haplotypes in this experiment, suggesting that the gene structure between DQA1 and DQB1 is conserved in these haplotypes.
从B淋巴母细胞系获得的基因组DNA用适当的限制性内切酶消化，并与几种针对编码HLA - DQ基因的探针杂交。用DQA1 3'非翻译（UT）区探针进行的Southern杂交在DR7DQ3单倍型中显示出DQ2型杂交模式。相反，DQB1 3'UT探针在相同单倍型中显示出DQ3型模式。基因克隆和DNA测序分析揭示了DR7DQ3单倍型中DQA1和DQB1基因之间的一个重复序列（TG）19。这些结果表明在单倍型DR7DQ3中，在这个潜在的Z - DNA结构附近发生了一次重组事件。在本实验中，DQA1和DQB1基因的3'UT区探针未能检测到DR4DQ3和DR4DQ4单倍型之间的限制性片段长度多态性（RFLP）差异，这表明在这些单倍型中DQA1和DQB1之间的基因结构是保守的。
