Satyanarayana K, Strominger J L
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Immunogenetics. 1992;35(4):235-40. doi: 10.1007/BF00166828.
The molecular organization of HLA-DQ regions derived from DR7, DQw2, and DR4, DQw3 parental haplotypes and DR7, DQw3, a presumed recombinant haplotype, have been studied to define the sequences between DQA1 and DQB1 which may have been involved in this recombinational event. The breakpoint was localized in the intergenic region near the 3' end of the DQB1 gene by restriction mapping. DNA sequences in the immediate vicinity of the breakpoint in DR7, DQw2 (parental), and DR7, DQw3 (recombinant) haplotypes revealed the presence of (CA)22 repeats, minisatellite-related sequences and GC-rich sequences. The intergenic regions varied considerably depending on the haplotype and contained several additional types of repetitive sequences including Alu and LINE repeats. Some of these sequences are related to sequences previously suggested to be involved in meiotic or somatic recombination. In particular, (CA)n repeats, which can adopt the Z-DNA conformation, have previously been shown to promote recombination in several systems.
已对源自DR7、DQw2和DR4、DQw3亲本单倍型以及DR7、DQw3（一种推测的重组单倍型）的HLA - DQ区域的分子组织进行了研究，以确定DQA1和DQB1之间可能参与了这一重组事件的序列。通过限制性图谱分析，将断点定位在DQB1基因3'端附近的基因间区域。DR7、DQw2（亲本）和DR7、DQw3（重组）单倍型中紧邻断点的DNA序列显示存在(CA)22重复序列、微卫星相关序列和富含GC的序列。基因间区域根据单倍型有很大差异，并且包含几种额外类型的重复序列，包括Alu和LINE重复序列。其中一些序列与先前认为参与减数分裂或体细胞重组的序列相关。特别是，能够采用Z - DNA构象的(CA)n重复序列，先前已在多个系统中显示出促进重组的作用。
