POST F J, KRISHNAMURTY G B, FLANAGAN M D
Appl Microbiol. 1963 Sep;11(5):430-5. doi: 10.1128/am.11.5.430-435.1963.
Sodium hexametaphosphate (HEX), the solvent of calcium alginate wool used in swabbing inanimate surfaces was studied relative to its effect on various bacterial populations, both pure cultures and wild. It was found that bacteria in wild populations were greatly inhibited, and that a percentage reduction of count was directly related to concentration of HEX. Most gram-positive bacteria were prevented from growing on a medium containing 0.1% HEX. This, or a higher concentration, occurred in the final medium when the method recommended in Standard Methods for the Examination of Dairy Products was followed. Growth of Sarcina lutea occurred on media with higher concentrations than that of inhibition (0.05%), if MgSO(4).7H(2)O was incorporated in the medium. Gram-negative bacteria were capable of growing in higher concentrations, even up to 10% HEX. A large percentage of the cells of some strains (represented by Pseudomonas fluorescens) were lysed on contact with HEX. Lysis could be prevented by the addition of NaCl or MgSO(4).7H(2)O. The evidence presented suggests that HEX, a phosphate-glass water-softening sequestrant, interferes with divalent cation metabolism, notably magnesium ion, and possibly others, producing cell division inhibition and loss of cell-wall integrity. The mechanism of action was not elucidated.
研究了用于擦拭无生命表面的海藻酸钙棉的溶剂六偏磷酸钠（HEX）对各种细菌群体（包括纯培养物和野生群体）的影响。结果发现，野生群体中的细菌受到极大抑制，且数量减少的百分比与HEX的浓度直接相关。大多数革兰氏阳性菌在含有0.1% HEX的培养基上无法生长。按照《乳制品检验标准方法》中推荐的方法操作时，在最终培养基中会出现这种或更高的浓度。如果在培养基中加入MgSO₄·7H₂O，藤黄八叠球菌在浓度高于抑制浓度（0.05%）的培养基上仍能生长。革兰氏阴性菌能够在更高浓度下生长，甚至高达10% HEX。一些菌株（以荧光假单胞菌为代表）的很大一部分细胞在与HEX接触时会裂解。加入NaCl或MgSO₄·7H₂O可防止裂解。所提供的证据表明，HEX作为一种磷酸盐玻璃水软化螯合剂，会干扰二价阳离子代谢，尤其是镁离子，可能还包括其他离子，从而导致细胞分裂抑制和细胞壁完整性丧失。其作用机制尚未阐明。
