Gaziev A I, Kutsyĭ M P, Zakrzhevskaia D T
Mol Biol (Mosk). 1992 May-Jun;26(3):532-9.
Histone H2A, H2B, and H1--specific proteinases tightly associated with histones were shown to be present in rat thymus nuclei. The activity of proteinases tightly associated with histones increases after exposure of animals to gamma-rays. The denatured DNA activated the histone H1-specific proteinase. These proteinase dissociated from histones in the presence of dithiothreitol. The histones and proteinases were divided into fractions by chromatography on DEAE-cellulose in the presence of 5 mM dithiothreitol.
已证明与组蛋白紧密相关的组蛋白H2A、H2B和H1特异性蛋白酶存在于大鼠胸腺细胞核中。动物暴露于γ射线后，与组蛋白紧密相关的蛋白酶活性增加。变性DNA激活了组蛋白H1特异性蛋白酶。在二硫苏糖醇存在下，这些蛋白酶与组蛋白分离。在5 mM二硫苏糖醇存在下，通过DEAE-纤维素柱层析将组蛋白和蛋白酶分离成不同组分。
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