LUNDGREN D G, BOTT K F
J Bacteriol. 1963 Sep;86(3):462-72. doi: 10.1128/jb.86.3.462-472.1963.
Lundgren, D. G. (Syracuse University, Syracuse, N.Y.) and K. F. Bott. Growth and sporulation characteristics of an organic sulfur-requiring auxotroph of Bacillus cereus. J. Bacteriol. 86:462-472. 1963.-This paper reports investigations of several aspects of growth and sporulation of an organic sulfur-requiring auxotroph of Bacillus cereus ATCC 4342. The wild type and B. cereus T were also studied for comparative purposes. Growth of the mutant on minimal medium plus methionine was normal, but sporulation was completely inhibited. Some reaction involved in vegetative-cell maturity was probably blocked at a point just prior to the "triggering" of sporulation, since abnormally large amounts of poly-beta-hydroxybutyric acid (PHB) were formed. Growth of the mutant in the presence of cystine or cysteine was also accompanied by the build-up of large amounts of PHB, but some endospores were formed (approximately 5% by 72 hr). Results of dipicolinic acid (DPA), calcium, and heat-resistance studies revealed that the few spores formed by the auxotrophic mutant when grown on cysteine were somewhat below wild-type strains in their development of heat resistance, and considerably lower in content of Ca and DPA. This was not the case with spores formed in cells grown on cystine; heat resistance compared favorably with wild-type spores, but the Ca and DPA levels were lower.
伦德格伦，D.G.（纽约州锡拉丘兹市锡拉丘兹大学）和K.F.博特。蜡样芽孢杆菌一种有机硫需求型营养缺陷型菌株的生长和芽孢形成特性。《细菌学杂志》86:462 - 472。1963年。——本文报道了对蜡样芽孢杆菌ATCC 4342的一种有机硫需求型营养缺陷型菌株生长和芽孢形成几个方面的研究。为作比较，还研究了野生型和蜡样芽孢杆菌T。该突变体在加有蛋氨酸的基本培养基上生长正常，但芽孢形成完全受到抑制。营养细胞成熟过程中涉及的某种反应可能在芽孢形成“触发”前的某个点被阻断，因为形成了异常大量的聚-β-羟基丁酸（PHB）。该突变体在胱氨酸或半胱氨酸存在下生长时也伴随着大量PHB的积累，但形成了一些内生孢子（72小时时约为5%）。吡啶二羧酸（DPA）、钙和耐热性研究结果表明，该营养缺陷型突变体在半胱氨酸上生长时形成的少数孢子在耐热性发育方面略低于野生型菌株，钙和DPA含量则低得多。在胱氨酸上生长的细胞中形成的孢子情况并非如此；耐热性与野生型孢子相当，但钙和DPA水平较低。
