Megraw R E, Beers R J
J Bacteriol. 1964 May;87(5):1087-93. doi: 10.1128/jb.87.5.1087-1093.1964.
Megraw, Robert E. (Iowa State University, Ames), and Russell J. Beers. Glyoxylate metabolism in growth and sporulation of Bacillus cereus. J. Bacteriol. 87:1087-1093. 1964.-Isocitrate lyase and malate synthetase were found in cell-free extracts of Bacillus cereus T. The patterns of synthesis of enzymes of the glyoxylic acid cycle were dependent upon the medium in which the organism was grown. Cells grown in acetate or in an acetate precursor, such as glucose, produced enzymes of the glyoxylic acid cycle in greatly diminished quantities, as compared with cells grown in media containing glutamate or yeast extract as principal carbon sources. Glutamate-grown cells had high isocitrate lyase activity but very low malate synthetase activity. Glyoxylate produced in this situation is metabolized by alternate pathways: conversion to tartronic semialdehyde and the latter to glyceric acid, thus providing evidence for a glycerate pathway; and reduction to glycolate (the reverse of this reaction was present at a low rate). Enzymatic activity of the glyoxylic acid cycle declines at the point where sporogenesis begins, indicating a metabolic shift for the synthesis of spore material.
梅格劳，罗伯特·E.（衣阿华州立大学，艾姆斯），以及拉塞尔·J.比尔斯。蜡样芽孢杆菌生长和孢子形成过程中的乙醛酸代谢。《细菌学杂志》87:1087 - 1093。1964年。——在蜡样芽孢杆菌T的无细胞提取物中发现了异柠檬酸裂解酶和苹果酸合成酶。乙醛酸循环酶的合成模式取决于该生物体生长的培养基。与在以谷氨酸或酵母提取物作为主要碳源的培养基中生长的细胞相比，在乙酸盐或乙酸盐前体（如葡萄糖）中生长的细胞产生的乙醛酸循环酶的量大大减少。在谷氨酸中生长的细胞具有高异柠檬酸裂解酶活性，但苹果酸合成酶活性非常低。在这种情况下产生的乙醛酸通过其他途径代谢：转化为酒石酸半醛，后者再转化为甘油酸，从而为甘油酸途径提供了证据；以及还原为乙醇酸（该反应的逆反应以低速率存在）。乙醛酸循环的酶活性在孢子形成开始时下降，表明代谢发生转变以合成孢子物质。
