刀豆氨酸对粪链球菌液化变种的生长刺激作用
GROWTH STIMULATION OF STREPTOCOCCUS FAECALIS VAR. LIQUEFACIENS BY CANAVANINE.
作者信息
HAMMEL J M, ZIMMERMAN L N
出版信息
J Bacteriol. 1963 Sep;86(3):490-3. doi: 10.1128/jb.86.3.490-493.1963.
Hammel, Jay M. (The Pennsylvania State University, University Park) and L. N. Zimmerman. Growth stimulation of Streptococcus faecalis var. liquefaciens by canavanine. J. Bacteriol. 86:490-493. 1963.-l-Canavanine, a competitive inhibitor of arginine, was found to stimulate the growth of Streptococcus faecalis var. liquefaciens in the presence of arginine. This growth stimulation by canavanine was unique, since it previously was found to inhibit the growth of organisms which require arginine. When equimolar concentrations of arginine and canavanine were tested from 10(-7) to 10(-2)m, maximal stimulation occurred at 10(-4)m. Canavanine concentrations of 5 x 10(-6)m to 10(-2)m stimulated growth in the presence of 5 x 10(-5)m arginine. Ornithine similarly stimulated growth. Canavanine inhibited the rate of arginine degradation by the arginine dihydrolase enzyme system in intact cells. It is assumed that the mechanism of canavanine stimulation involves an inhibition of arginine desimidase of the arginine dihydrolase enzyme system, which in turn makes more arginine available for growth over a longer period of time.
哈默尔，杰伊·M.（宾夕法尼亚州立大学，帕克分校）和L.N.齐默尔曼。刀豆氨酸对粪链球菌液化变种的生长刺激作用。《细菌学杂志》86:490 - 493。1963年。发现L - 刀豆氨酸，一种精氨酸的竞争性抑制剂，在有精氨酸存在的情况下能刺激粪链球菌液化变种的生长。刀豆氨酸的这种生长刺激作用很独特，因为之前发现它能抑制需要精氨酸的生物体的生长。当从10⁻⁷到10⁻²摩尔浓度对等摩尔浓度的精氨酸和刀豆氨酸进行测试时，在10⁻⁴摩尔浓度时出现最大刺激作用。在存在5×10⁻⁵摩尔浓度精氨酸的情况下，5×10⁻⁶摩尔浓度到10⁻²摩尔浓度的刀豆氨酸能刺激生长。鸟氨酸也同样能刺激生长。刀豆氨酸抑制完整细胞中精氨酸二水解酶系统对精氨酸的降解速率。据推测，刀豆氨酸刺激生长的机制涉及对精氨酸二水解酶系统中精氨酸脱亚氨酶的抑制，这反过来又使更多精氨酸在更长时间内可用于生长。
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