粪链球菌液化变种中基因转移的新模式。
NEW MODE OF GENETIC TRANSFER IN STREPTOCOCCUS FAECALIS VAR. LIQUEFACIENS.
作者信息
RAYCROFT R E, ZIMMERMAN L N
出版信息
J Bacteriol. 1964 Apr;87(4):799-801. doi: 10.1128/jb.87.4.799-801.1964.
Raycroft, Ruth E. (The Pennsylvania State University, University Park), and L. N. Zimmerman. New mode of genetic transfer in Streptococcus faecalis var. liquefaciens. J. Bacteriol. 87:799-801. 1964.-When chloramphenicol-resistant mutants are grown in mixed culture with the sensitive wild type of Streptococcus faecalis var. liquefaciens 31, a transfer of chloramphenicol resistance occurs. (The marker is stable through at least five transfers.) The transfer mechanism is not inhibited by deoxyribonuclease, nor is it phage-mediated; genetic transfer is apparently dependent upon cell contact. Although kinetic studies have yet to be completed, preliminary data indicate a high efficiency (2.2 per donor cell in some experiments) of transmission. Two levels of drug resistance, 7 and 50 mug, were transferred; in each case, recipient cells expressed the donor's degree of resistance.
雷克罗夫特，露丝·E.（宾夕法尼亚州立大学，帕克校区），以及L. N. 齐默尔曼。粪肠球菌液化变种的新基因转移模式。《细菌学杂志》87:799 - 801。1964年。——当耐氯霉素突变体与粪肠球菌液化变种31的敏感野生型混合培养时，会发生氯霉素抗性的转移。（该标记至少经过五次传代仍稳定。）转移机制不受脱氧核糖核酸酶抑制，也不是噬菌体介导的；基因转移显然依赖于细胞接触。尽管动力学研究尚未完成，但初步数据表明转移效率很高（在某些实验中每个供体细胞为2.2）。转移了7微克和50微克两种耐药水平；在每种情况下，受体细胞都表现出供体的耐药程度。
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