辛酸钠对白色念珠菌的作用。II. 不同浓度对生化变化的影响。
EFFECTS OF SODIUM CAPRYLATE ON CANDIDA ALBICANS. II. INFLUENCE OF VARIOUS CONCENTRATIONS ON BIOCHEMICAL CHANGES.
作者信息
PAYNE W J, BANNISTER E R
出版信息
J Bacteriol. 1963 Sep;86(3):558-62. doi: 10.1128/jb.86.3.558-562.1963.
Payne, W. J. (University of Georgia, Athens) and E. R. Bannister. Effects of sodium caprylate on Candida albicans. II. Influence of various concentrations on biochemical changes. J. Bacteriol. 86:558-562. 1963.-Suspending resting cells of Candida albicans in buffered solutions of 0.1 m sodium caprylate resulted in leakage of K(+), protein, ribonucleic acid (RNA), and carbohydrate (including glucose) but not of deoxyribonucleic acid (DNA) or sterol. In 0.025 m caprylate, the yeast leaked carbohydrate but not any of the other metabolites, whereas at 2.5 x 10(-3)m or in buffer alone there was no significant leakage. Inclusion of 2.5 x 10(-3)m sodium caprylate in Sabouraud dextrose broth cultures, however, suppressed growth greatly. Assays of relative protein, RNA, and DNA contents of dried cells taken from the suppressed cultures revealed changes in the rates of production of each from those of untreated cultures.
佩恩，W. J.（佐治亚大学，雅典）和E. R. 班尼斯特。辛酸钠对白色念珠菌的影响。II. 不同浓度对生化变化的影响。《细菌学杂志》86:558 - 562。1963年。——将白色念珠菌的静止细胞悬浮于0.1 m辛酸钠的缓冲溶液中，导致钾离子（K⁺）、蛋白质、核糖核酸（RNA）和碳水化合物（包括葡萄糖）泄漏，但脱氧核糖核酸（DNA）或固醇未泄漏。在0.025 m辛酸钠中，酵母细胞泄漏碳水化合物，但未泄漏其他任何代谢物，而在2.5×10⁻³ m辛酸钠中或仅在缓冲液中时，没有明显的泄漏。然而，在沙氏葡萄糖肉汤培养物中加入2.5×10⁻³ m辛酸钠会极大地抑制生长。对从受抑制培养物中获取的干燥细胞的相对蛋白质、RNA和DNA含量进行测定，结果显示与未处理培养物相比，每种物质的产生速率都发生了变化。
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