WELKER N E, CAMPBELL L L
J Bacteriol. 1963 Oct;86(4):681-6. doi: 10.1128/jb.86.4.681-686.1963.
Welker, N. E. (Western Reserve University, Cleveland, Ohio) and L. Leon Campbell. Effect of carbon sources on formation of alpha-amylase by Bacillus stearothermophilus. J. Bacteriol. 86:681-686. 1963.-A chemically defined medium was devised for use in alpha-amylase induction studies. The addition of 0.1% casein hydrolysate to the chemically defined medium permitted growth on fructose, and with glucose, sucrose, maltose, starch, and glycerol it shortened the lag period and increased both the growth rate and the total enzyme produced. Growth did not occur when gluconate, acetate, or succinate were used as carbon sources. alpha-Amylase was produced during the logarithmic phase of growth; the amount produced was inversely proportional to the rate of growth. The poorer the carbon source for growth (glycerol, k = 0.24; glucose, k = 0.26; sucrose, k = 0.42), the higher was the amount of enzyme produced (glycerol, 109 units/ml; glucose, 103 units/ml; sucrose, 45 units/ml). Cells grown on technical-grade maltose (k = 0.26) or starch (k = 0.42) did not conform to this relationship in that unusually large amounts of alpha-amylase were produced (362 and 225 units/ml, respectively). Cells grown on fructose or sucrose had the same growth rate (k = 0.42), but smaller amounts of alpha-amylase were produced on fructose (fructose, 0 to 4 units/ml; sucrose, 45 units/ml). An intracellular alpha-amylase was not detected in Bacillus stearothermophilus.
韦尔克，N. E.（俄亥俄州克利夫兰市西部保留地大学）和L. 利昂·坎贝尔。碳源对嗜热脂肪芽孢杆菌α-淀粉酶形成的影响。《细菌学杂志》86:681 - 686。1963年。——设计了一种化学成分明确的培养基用于α-淀粉酶诱导研究。向化学成分明确的培养基中添加0.1%的酪蛋白水解物可使细菌在果糖上生长，而对于葡萄糖、蔗糖、麦芽糖、淀粉和甘油，它缩短了延迟期，并提高了生长速率和总酶产量。当使用葡萄糖酸盐、乙酸盐或琥珀酸盐作为碳源时，细菌无法生长。α-淀粉酶在生长的对数期产生；产生的量与生长速率成反比。作为生长碳源越差（甘油，k = 0.24；葡萄糖，k = 0.26；蔗糖，k = 0.42），产生的酶量越高（甘油，109单位/毫升；葡萄糖，103单位/毫升；蔗糖，45单位/毫升）。在工业级麦芽糖（k = 0.26）或淀粉（k = 0.42）上生长的细胞不符合这种关系，因为产生了异常大量的α-淀粉酶（分别为362和225单位/毫升）。在果糖或蔗糖上生长的细胞具有相同的生长速率（k = 0.42），但在果糖上产生的α-淀粉酶量较少（果糖，0至4单位/毫升；蔗糖，45单位/毫升）。在嗜热脂肪芽孢杆菌中未检测到细胞内α-淀粉酶。
