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嗜热脂肪芽孢杆菌在分批培养和恒化器培养中α-淀粉酶的产生。

The production of alpha-amylase in batch and chemostat culture by Bacillus stearothermophilus.

作者信息

Davis P E, Cohen D L, Whitaker A

出版信息

Antonie Van Leeuwenhoek. 1980;46(4):391-8. doi: 10.1007/BF00421985.

DOI:10.1007/BF00421985
PMID:6160812
Abstract

The production of alpha-amylase (alpha-1,4-glucan 4-glucanohydrolase; EC 3.2.1.1) by a strain of Bacillus stearothermophilus isolated from leaf litter was investigated in a tryptone-maltose medium at 55 degrees C in batch and chemostat culture. Amylase production was growth-limited and restricted to the exponential phase in batch culture. The enzyme yield was reduced by 40% when the culture pH was maintained at pH 7.2. Amylase production in chemostat culture was influenced by the growth rate throughout the dilution rate range used.

摘要

对从落叶层分离出的嗜热脂肪芽孢杆菌菌株在胰蛋白胨-麦芽糖培养基中于55℃进行分批培养和恒化器培养时α-淀粉酶(α-1,4-葡聚糖4-葡聚糖水解酶;EC 3.2.1.1)的产生情况进行了研究。在分批培养中,淀粉酶的产生受生长限制,且仅限于指数生长期。当培养物pH维持在7.2时,酶产量降低了40%。在整个使用的稀释率范围内,恒化器培养中的淀粉酶产生受生长速率的影响。

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引用本文的文献

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Microb Cell Fact. 2019 Apr 11;18(1):69. doi: 10.1186/s12934-019-1119-8.
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Continuous Production of Thermostable beta-Amylase with Clostridium thermosulfurogenes: Effect of Culture Conditions and Metabolite Levels on Enzyme Synthesis and Activity.利用嗜热脱硫梭菌连续生产热稳定β-淀粉酶:培养条件和代谢物水平对酶合成和活性的影响。
Appl Environ Microbiol. 1989 Mar;55(3):689-94. doi: 10.1128/aem.55.3.689-694.1989.

本文引用的文献

1
EFFECT OF CARBON SOURCES ON FORMATION OF ALPHA-AMYLASE BY BACILLUS STEAROTHERMOPHILUS.碳源对嗜热脂肪芽孢杆菌产生α-淀粉酶的影响
J Bacteriol. 1963 Oct;86(4):681-6. doi: 10.1128/jb.86.4.681-686.1963.
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Studies on alpha-amylase formation by Bacillus subtilis.枯草芽孢杆菌产生α-淀粉酶的研究。
Biochem J. 1962 May;83(2):256-63. doi: 10.1042/bj0830256.
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The extracellular alpha-amylase of bacillus stearothermophilus.嗜热脂肪芽孢杆菌的胞外α-淀粉酶
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Microbial amylases.微生物淀粉酶
Adv Appl Microbiol. 1965;7:273-304. doi: 10.1016/s0065-2164(08)70389-7.
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Comparison of the alpha-amylase of Bacillus subtilis and Bacillus amyloliquefaciens.枯草芽孢杆菌和解淀粉芽孢杆菌α-淀粉酶的比较。
J Bacteriol. 1967 Oct;94(4):1131-5. doi: 10.1128/jb.94.4.1131-1135.1967.
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Continuous culture studies on the biosynthesis of alkaline protease, neutral protease and -amylase by Bacillus subtilis NRRL-B3411.枯草芽孢杆菌NRRL-B3411碱性蛋白酶、中性蛋白酶和α-淀粉酶生物合成的连续培养研究
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Mutation of Bacillus subtilis causing hyperproduction of alpha-amylase and protease, and its synergistic effect.枯草芽孢杆菌的突变导致α-淀粉酶和蛋白酶的过量产生及其协同效应。
J Bacteriol. 1975 Oct;124(1):48-54. doi: 10.1128/jb.124.1.48-54.1975.
8
Extracellular enzyme synthesis in the genus Bacillus.芽孢杆菌属中的胞外酶合成
Bacteriol Rev. 1977 Sep;41(3):711-53. doi: 10.1128/br.41.3.711-753.1977.