LEITNER F, SWEENEY H M, MARTIN T F, COHEN S
J Bacteriol. 1963 Oct;86(4):717-27. doi: 10.1128/jb.86.4.717-727.1963.
Leitner, Felix (Michael Reese Hospital and Medical Center, Chicago, Ill.), Helen M. Sweeney, T. F. Martin, and Sidney Cohen. Induction of staphylococcal penicillinase by benzylpenicillin: effect of pH, concentration of ferrous ion and inducer, and duration of exposure of cells to inducer. J. Bacteriol. 86:717-727. 1963.-The kinetics of induction of penicillinase by benzylpenicillin in exponentially multiplying Staphylococcus aureus strain 55-C-1 were shown to vary with the pH. At pH 7.3 in the absence of free inducer, the rate of increase of penicillinase activity rapidly declined and came to a halt. At pH 5.4 to 5.5 and in the presence of optimal concentrations of Fe(++), the penicillinase activity of the induced culture increased linearly with time for 2.5 or more generations, but the rate of increase usually declined eventually. Evidence was advanced to support the concept that the acidic pH and optimal Fe(++) concentration maintain the induced formation of enzyme. The induced increase in penicillinase activity appeared 3 to 4 min after the addition of benzylpenicillin. The degree of induction of penicillinase varied with the duration of exposure of the staphylococci to the inducer and with the concentration of inducer. Maximal induction under our conditions was attained by exposure for 15 min to benzyl-penicillin at an initial concentration between 0.6 and 2 units/ml.
莱特纳，费利克斯（迈克尔·里斯医院及医疗中心，伊利诺伊州芝加哥），海伦·M·斯威尼，T·F·马丁，以及西德尼·科恩。苄青霉素诱导葡萄球菌青霉素酶的产生：pH值、亚铁离子浓度和诱导剂浓度以及细胞暴露于诱导剂的持续时间的影响。《细菌学杂志》86:717 - 727。1963年。——研究表明，在指数生长的金黄色葡萄球菌55 - C - 1菌株中，苄青霉素诱导青霉素酶产生的动力学随pH值变化。在pH 7.3且无游离诱导剂的情况下，青霉素酶活性的增加速率迅速下降并停止。在pH 5.4至5.5且存在最佳浓度的Fe(++)时，诱导培养物的青霉素酶活性在2.5代或更多代的时间内随时间呈线性增加，但增加速率最终通常会下降。有证据支持酸性pH值和最佳Fe(++)浓度维持酶的诱导形成这一概念。添加苄青霉素后3至4分钟出现诱导的青霉素酶活性增加。青霉素酶的诱导程度随葡萄球菌暴露于诱导剂的持续时间以及诱导剂浓度而变化。在我们的条件下，通过将葡萄球菌暴露于初始浓度为0.6至2单位/毫升的苄青霉素15分钟可达到最大诱导效果。
