COHEN S, SWEENEY H, LEITNER F
Science. 1965 Aug 20;149(3686):877-9. doi: 10.1126/science.149.3686.877.
In eight highly inducible strains of Staphylococcus aureus repression of the formation of penicillinase was temperature-sensitive under conditions suggesting direct thermal inactivation of the repressor. Restoration of repression required protein synthesis. These strains were resistant to benzylpenicillin and to many other antibiotics. One auxotrophic mutant had gredtly reduced temperature sensitivity but was still normally inducible. Six strains were relatively poorly inducible, exhibited a proportionately smaller increase in enzyme after exposure to elevated temperature, and were sensitive to antibiotics other than benzylpenicillin. Temperature sensitivity may be a useful character in studies of the physiology and genetics of the repression of staphylococcal penicillinase.
在8株高度可诱导的金黄色葡萄球菌中，在提示阻遏物直接热失活的条件下，青霉素酶形成的阻遏是温度敏感的。阻遏的恢复需要蛋白质合成。这些菌株对苄青霉素和许多其他抗生素具有抗性。一个营养缺陷型突变体的温度敏感性大大降低，但仍可正常诱导。6株菌株的诱导性相对较差，在暴露于高温后酶的增加比例较小，并且对苄青霉素以外的抗生素敏感。温度敏感性可能是研究葡萄球菌青霉素酶阻遏的生理学和遗传学的一个有用特性。
