el-Sayed G N, Ignoffo C M, Leathers T D, Gupta S C
Biological Control of Insects Research Laboratory, USDA/ARS, Columbia, MO 65205.
Mycopathologia. 1992 Apr;118(1):29-36. doi: 10.1007/BF00472569.
An apiZYM system, with 19 substrates, was used to detect enzymes expressed by germinating conidia of Nomuraea rileyi (5 isolates), Nomuraea atypicola, Nomuraea anemonoides, Beauveria bassiana and Metarhizium anisopliae. Similar enzyme profiles were obtained for two of the N. rileyi isolates (Mississippi, Ecuador) regardless of whether culture medium (Sabouraud-maltose-yeast) or cuticle (from larvae of Trichoplusia ni, Heliothis zea or Heliothis virescens) were used as substrates. Centroid-clustering analysis revealed three distinct enzyme profiles.
使用一种含有19种底物的apiZYM系统来检测莱氏野村菌（5个分离株）、异形野村菌、银莲花野村菌、球孢白僵菌和绿僵菌的萌发分生孢子所表达的酶。无论使用培养基（沙氏麦芽糖酵母培养基）还是角质层（来自粉纹夜蛾、棉铃虫或烟芽夜蛾的幼虫）作为底物，莱氏野村菌的两个分离株（密西西比州、厄瓜多尔）都获得了相似的酶谱。质心聚类分析揭示了三种不同的酶谱。
