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φX174的感染过程：核酸外切酶对复制型的影响

PROCESS OF INFECTION WITH PHI-X174: EFFECT OF EXONUCLEASES ON THE REPLICATIVE FORM.

作者信息

BURTON A, SINSHEIMER R L

出版信息

Science. 1963 Nov 15;142(3594):962-3. doi: 10.1126/science.142.3594.962.

DOI:10.1126/science.142.3594.962
PMID:14069225
Abstract

When a preparation containing the DNA of the bacteriophage øX174 in its replicative form together with DNA from Escherichia coli is treated with exonucleases, the replicative form retains its characteristic physical properties, while the bacterial DNA is degraded. The evidence suggests that the mature replicative form exists as a closed ring.

摘要

当用外切核酸酶处理一种含有复制形式的噬菌体øX174的DNA以及来自大肠杆菌的DNA的制剂时，复制形式保留其特征性物理性质，而细菌DNA则被降解。证据表明成熟的复制形式以闭环形式存在。

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