噬菌体φX174的复制型II DNA；对大肠杆菌DNA聚合酶核酸外切酶切割的抗性
Replicative form II DNA of phi X174; resistance to exonucleolytic cleavage by E. coli DNA polymerase.
作者信息
Razin A, Sinsheimer R L
出版信息
Proc Natl Acad Sci U S A. 1970 Jul;66(3):646-50. doi: 10.1073/pnas.66.3.646.
Abstract
The progeny replicative form II DNA isolated from E. coli cells infected with varphiX174 is resistant to the exonucleolytic activity associated with E. coli DNA polymerase. A limited endonucleolytic cleavage with micrococcal endonuclease renders the replicative form II molecule susceptible to the exonucleolytic activity associated with the E. coli DNA polymerase. These experiments suggest that the resistance of the replicative form II DNA to the exonucleolytic activity is a result of the structure of these molecules at their 5'-terminus.
摘要
从感染了φX174的大肠杆菌细胞中分离出的子代复制型II DNA对与大肠杆菌DNA聚合酶相关的核酸外切酶活性具有抗性。用微球菌核酸内切酶进行有限的内切核酸酶切割，可使复制型II分子对与大肠杆菌DNA聚合酶相关的核酸外切酶活性敏感。这些实验表明，复制型II DNA对核酸外切酶活性的抗性是这些分子5'-末端结构的结果。
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本文引用的文献
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Studies on the mechanism of action of micrococcal nuclease. 1. Degradation of thymus deoxyribonucleic acid.微球菌核酸酶作用机制的研究。1. 胸腺脱氧核糖核酸的降解
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