BELLER F K, DEBROVNER C H, DOUGLAS G W
J Exp Med. 1963 Aug 1;118(2):245-56. doi: 10.1084/jem.118.2.245.
The intravenous injection of endotoxin in human plasma into rabbits produces a marked potentiation of lethal effect, when compared to the mortality associated with comparable doses of endotoxin in saline alone. A similar enhancement was noted with other heterologous plasma (guinea pig, rat) but not with homologous plasma. The potentiating factor is not in the albumin or gamma globulin fractions, is not concerned with fibrinogen, and is heat-labile. Tolerance of the recipient animal to endotoxin destroys the lethal effect. Within a period of 3 hours, endotoxin and human plasma may be administered separately, without regard to timing or sequence, without loss of the lethal effect. The enhancement of lethal effect is not avoided by pretreatment with heparin or cortisone. Preliminary experiments indicate that the loss of lethal effect found after incubation of endotoxin-plasma mixtures may be due to a separate inhibitor, but is not due to loss of the potentiating factor.
与单独使用等剂量内毒素生理盐水相比，将人血浆中的内毒素静脉注射到兔子体内会产生显著的致死效应增强。在其他异种血浆（豚鼠、大鼠）中也观察到类似的增强作用，但同源血浆则没有。增强因子不在白蛋白或γ球蛋白部分，与纤维蛋白原无关，且对热不稳定。受体动物对内毒素的耐受性会破坏致死效应。在内毒素和人血浆可以在3小时内分别给药，而无需考虑给药时间或顺序，且不会丧失致死效应。用肝素或可的松预处理并不能避免致死效应的增强。初步实验表明，内毒素-血浆混合物孵育后发现的致死效应丧失可能是由于一种单独的抑制剂，但不是由于增强因子的丧失。
