SKARNES R C, ROSEN F S, SHEAR M J, LANDY M
J Exp Med. 1958 Nov 1;108(5):685-99. doi: 10.1084/jem.108.5.685.
A humoral substance which inactivates endotoxin in vitro has been shown to be clearly distinguishable from complement, properdin, and specific antibody. For the present, it is designated "endotoxin-detoxifying component" or EDC. Animal species could be grouped in three categories with regard to the EDC activity of their sera; rat serum was highly potent; chimpanzee, dog, horse, and guinea pig sera were much less active; mouse, rabbit, and sheep sera exhibited no activity. The EDC potency of human sera varied widely, ranging from high to barely discernible activity. In contrast to the variations of EDC potency in serum, citrated plasma from all species manifested high potency of about the same magnitude. The influence of time, temperature, pH, and concentration of reactants on the inactivation of endotoxin by EDC was examined. EDC activity in plasma and serum was found to be labile to beating at 56 degrees C. for 1 hour. Bacterial endotoxins, derived by different isolation procedures from smooth and rough Gram-negative species, varied considerably in susceptibility to EDC action.
一种能在体外使内毒素失活的体液物质已被证明与补体、备解素和特异性抗体明显不同。目前,它被命名为“内毒素解毒成分”或EDC。就血清的EDC活性而言,动物物种可分为三类;大鼠血清活性很高;黑猩猩、狗、马和豚鼠血清的活性则低得多;小鼠、兔子和绵羊血清没有活性。人血清的EDC活性差异很大,从高到几乎难以察觉的活性都有。与血清中EDC活性的变化相反,所有物种的枸橼酸化血浆都表现出大致相同程度的高活性。研究了时间、温度、pH值和反应物浓度对EDC使内毒素失活的影响。发现血浆和血清中的EDC活性在56℃加热1小时后不稳定。通过不同分离程序从光滑型和粗糙型革兰氏阴性菌中获得的细菌内毒素对EDC作用的敏感性差异很大。