Nitta T, Allegretta M, Okumura K, Sato K, Steinman L
Department of Neurology and Genetics, Stanford University School of Medicine.
Neurosurg Rev. 1992;15(3):203-7. doi: 10.1007/BF00345934.
Expression of lymphokine genes in the human astroglial cell lineage was studied. Primers for 9 different human lymphokines, from IL-1 alpha to IL-8, were used to analyze RNA transcripts in 5 cultured human astrocytoma cell lines and fresh brain specimens by PCR. mRNA transcripts for IL-8 were detected in all neuroglial cells. In addition to the cultured cells, we examined IL-8 gene expression within human malignant astrocytoma, peritumoral brain and autopsied normal brains. The result shows that tumor and cells of the surrounding reactive lesion express IL-8 genes, but it is not expressed in normal brains. Next, the concentration of IL-8 in supernatants of cultured cells was measured quantitatively by a solid phase ELISA assay. IL-8 activity was produced constitutively in all astrocytomas and increased markedly upon stimulation with IL-1 beta or TNF alpha, in both a time- and dose-dependent fashion. From these results, it is suspected that astroglial cell-derived IL-8 may take part in neutrophil-mediated inflammation which accompanies infection, degeneration and malignancy in the brain.
对人星形胶质细胞系中淋巴因子基因的表达进行了研究。使用针对从白细胞介素-1α到白细胞介素-8的9种不同人类淋巴因子的引物，通过聚合酶链反应（PCR）分析5种培养的人星形细胞瘤细胞系和新鲜脑标本中的RNA转录本。在所有神经胶质细胞中均检测到白细胞介素-8的mRNA转录本。除了培养的细胞外，我们还检测了人恶性星形细胞瘤、肿瘤周围脑组织和尸检正常脑组织中白细胞介素-8基因的表达。结果显示，肿瘤及周围反应性病变的细胞表达白细胞介素-8基因，但在正常脑组织中不表达。接下来，通过固相酶联免疫吸附测定法定量测量培养细胞上清液中白细胞介素-8的浓度。在所有星形细胞瘤中均组成性产生白细胞介素-8活性，在用白细胞介素-1β或肿瘤坏死因子α刺激后，其活性以时间和剂量依赖性方式显著增加。从这些结果推测，星形胶质细胞衍生的白细胞介素-8可能参与了伴随脑部感染、退变和恶性肿瘤的中性粒细胞介导的炎症反应。
