Liu H, Llewellyn-Smith I J, Pilowsky P, Basbaum A I
Department of Anatomy, University of California, San Francisco 94143.
Neurosci Lett. 1992 Apr 13;138(1):183-7. doi: 10.1016/0304-3940(92)90501-w.
The synaptic relationships between gamma-aminobutyric acid (GABA)-immunoreactive and enkephalin-immunoreactive profiles in the cat spinal cord were examined using combined pre-embedding immunoperoxidase and post-embedding immunogold electron microscopic immunocytochemistry. Although colchicine was not used, enkephalin-immunoreactive somata and dendrites were detected in regions associated with nociceptive transmission, including laminae I, II, V and X. In each of these laminae, many GABA-immunoreactive terminals were found presynaptic to enkephalin-immunoreactive cell bodies and dendrites. We propose that disinhibition of opioid-containing neurons may be a common feature of pain-related circuits in the cat spinal cord.
利用联合的包埋前免疫过氧化物酶和包埋后免疫金电子显微镜免疫细胞化学技术，研究了猫脊髓中γ-氨基丁酸（GABA）免疫反应阳性和脑啡肽免疫反应阳性结构之间的突触关系。尽管未使用秋水仙碱，但在与伤害性感受传递相关的区域，包括I、II、V和X层，检测到了脑啡肽免疫反应阳性的胞体和树突。在这些层中的每一层，许多GABA免疫反应阳性终末位于脑啡肽免疫反应阳性细胞体和树突的突触前。我们提出，含阿片样物质神经元的去抑制可能是猫脊髓中与疼痛相关回路的一个共同特征。
