疲劳性等长收缩期间猫中脑导水管周围灰质中免疫反应性脑啡肽能物质的释放。

Release of immunoreactive enkephalinergic substances in the periaqueductal grey of the cat during fatiguing isometric contractions.

作者信息

Williams C A, Holtsclaw L I, Chiverton J A

机构信息

Department of Physiology, College of Medicine, East Tennessee State University, Johnson City 37614-0002.

出版信息

Neurosci Lett. 1992 May 11;139(1):19-23. doi: 10.1016/0304-3940(92)90848-2.

DOI:10.1016/0304-3940(92)90848-2

PMID:1407679

Abstract

Antibody-coated microprobes were used to determine whether immunoreactive enkephalins were released in response to fatiguing isometric contractions of the hind-limb muscles in cats anesthetized with alpha-chloralose. Contractions were performed by stimulating the tibial nerve via a microprocessor-controlled stimulator. Microprobes were inserted into the periaqueductal grey (P 0.5-1.0 mm) prior to, during and following fatiguing contractions. During fatiguing contractions, mean arterial blood pressure increased by 76 +/- 9 mmHg above resting and recovery levels. Levels of immunoreactive enkephalins were elevated in the dorsolateral periaqueductal grey during the isometric contraction when compared to resting levels. It is possible that isometric muscle contraction causes the release of Met-enkephalin-like substances in the periaqueductal grey.

摘要

抗体包被的微探针用于确定在用α-氯醛糖麻醉的猫中，免疫反应性脑啡肽是否会因后肢肌肉疲劳性等长收缩而释放。通过微处理器控制的刺激器刺激胫神经来进行收缩。在疲劳性收缩之前、期间和之后，将微探针插入导水管周围灰质（距表面0.5 - 1.0毫米）。在疲劳性收缩期间，平均动脉血压比静息和恢复水平升高了76±9毫米汞柱。与静息水平相比，等长收缩期间背外侧导水管周围灰质中的免疫反应性脑啡肽水平升高。等长肌肉收缩可能导致导水管周围灰质中释放甲硫氨酸脑啡肽样物质。