等长收缩期间猫脑干部位免疫反应性神经肽Y的释放。

Release of immunoreactive neuropeptide Y from brainstem sites in the cat during isometric contractions.

作者信息

Williams C A, Holtsclaw L I, Chiverton J A

机构信息

Department of Physiology, College of Medicine, East Tennessee State University, Johnson City 37614-0576.

出版信息

Neuropeptides. 1993 Jan;24(1):53-61. doi: 10.1016/0143-4179(93)90040-h.

DOI:10.1016/0143-4179(93)90040-h

PMID:8429923

Abstract

Fatiguing isometric contractions of the left hind-limb muscles in cats anesthetized with alpha-chloralose caused mean arterial pressure to increase by 82 +/- 18 mmHg above resting and post-contraction levels and heart rates to increase by 15 +/- 5 beats/min. Contractions were performed by stimulating the tibial nerve via a microprocessor-controlled stimulator. Glass microprobes, coated with antibody specific for neuropeptide Y (NPY) were inserted bilaterally into the periaqueductal grey (P 0.5-1.0, LR 2.0 mm) or into the right ventrolateral medulla (3.0 mm rostral to obex; LR 3.5 mm) prior to, during and following fatiguing contractions to determine whether immunoreactive NPY was released. No release of immunoreactive NPY was detected from the site in the ventrolateral medulla. Immunoreactive NPY was released from the contralateral but not the ipsilateral periaqueductal grey during the isometric contractions, suggesting that NPY-like substances maybe involved with the integration of muscle afferent input into this area of the brainstem.

摘要

用α-氯醛糖麻醉的猫，其左后肢肌肉进行疲劳性等长收缩时，平均动脉压比静息和收缩后水平升高82±18 mmHg，心率增加15±5次/分钟。收缩通过微处理器控制的刺激器刺激胫神经来进行。在疲劳性收缩之前、期间和之后，将涂有神经肽Y（NPY）特异性抗体的玻璃微探针双侧插入导水管周围灰质（P 0.5 - 1.0，LR 2.0 mm）或右侧腹外侧延髓（距闩前方3.0 mm；LR 3.5 mm），以确定免疫反应性NPY是否释放。未检测到腹外侧延髓部位有免疫反应性NPY释放。等长收缩期间，免疫反应性NPY从对侧而非同侧导水管周围灰质释放，这表明NPY样物质可能参与了肌肉传入输入到脑干该区域的整合过程。