Michikawa M, Kikuchi S, Kim S U
Department of Medicine, University of British Columbia, Vancouver, Canada.
Neurosci Lett. 1992 Jun 8;140(1):75-7. doi: 10.1016/0304-3940(92)90685-z.
The effects of leukemia inhibitory factor (LIF) on choline acetyltransferase (ChAT) enzyme activity in cultured mouse spinal cord neurons were examined. The administration of LIF to cultures at concentrations of 10 U/ml and higher enhanced ChAT activity approximately 3- to 4-fold in cultured spinal cord neurons. Among neurotrophic factors tested, basic fibroblast growth factor (bFGF) and insulin-like growth factor I (IGF-I) stimulated the development of ChAT activity but to a smaller extent than LIF, while interleukin 3 (IL-3), interleukin 6 (IL-6) and nerve growth factor (NGF) showed no apparent effect on ChAT development. Our results indicate that LIF, which has not been known to have any trophic effect on mammalian central nervous system neurons to date, acts as a potent differentiation factor for ChAT in cholinergic neurons of mouse spinal cord in culture.
研究了白血病抑制因子（LIF）对培养的小鼠脊髓神经元中胆碱乙酰转移酶（ChAT）活性的影响。以10 U/ml及更高浓度向培养物中添加LIF，可使培养的脊髓神经元中的ChAT活性增强约3至4倍。在所测试的神经营养因子中，碱性成纤维细胞生长因子（bFGF）和胰岛素样生长因子I（IGF-I）刺激了ChAT活性的发展，但程度小于LIF，而白细胞介素3（IL-3）、白细胞介素6（IL-6）和神经生长因子（NGF）对ChAT的发展无明显影响。我们的结果表明，迄今为止尚不知道对哺乳动物中枢神经系统神经元有任何营养作用的LIF，在培养的小鼠脊髓胆碱能神经元中作为ChAT的有效分化因子发挥作用。
