Kushima Y, Hatanaka H
Division of Protein Biosynthesis, Osaka University, Japan.
Neurosci Lett. 1992 Aug 31;143(1-2):110-4. doi: 10.1016/0304-3940(92)90244-2.
Interleukin-6 (IL-6) and leukemia inhibitory factor (LIF) promoted the survival of acetylcholinesterase (AChE)-positive neurons in culture from embryonic E15 rat spinal cord. Half of the AChE-positive neurons died during 3-7 days in culture in the absence of IL-6 and LIF. However, IL-6 at a concentration of 5 ng/ml completely prevented the death of AChE-positive neurons. LIF at a concentration of 5 U/ml also stimulated the survival of neurons, although to a lesser extent than IL-6. IL-6 and LIF also increased the numbers of process-bearing neuron-like cells in culture. The dose-dependencies of IL-6 and LIF with regard to the survival of total neuron-like cells were different from those for AChE-positive neurons.
白细胞介素-6（IL-6）和白血病抑制因子（LIF）可促进来自胚胎E15大鼠脊髓的培养物中乙酰胆碱酯酶（AChE）阳性神经元的存活。在没有IL-6和LIF的情况下，培养3至7天期间，一半的AChE阳性神经元死亡。然而，浓度为5 ng/ml的IL-6完全阻止了AChE阳性神经元的死亡。浓度为5 U/ml的LIF也刺激了神经元的存活，尽管程度低于IL-6。IL-6和LIF还增加了培养物中带有突起的神经元样细胞的数量。IL-6和LIF在总神经元样细胞存活方面的剂量依赖性与AChE阳性神经元的不同。
