TOBE B A
Can Med Assoc J. 1963 Nov 30;89(22):1124-32.
The spontaneous formation of ammonia which takes place when shed whole blood is allowed to stand was investigated and was found to consist of a complex series of reactions. The rate of ammonia formation was initially rapid but gradually slowed, and the maximum amount of ammonia was formed after a period of about seven days. Both the type of anticoagulant used and the availability of oxygen influenced the rate of ammonia formation. This reaction was inhibited when the blood was kept frozen but it was found that the measurement of the ammonia content of frozen and thawed blood was both difficult and inaccurate. Dilute solutions of zinc bromide partially inhibited this reaction.Compounds which give rise to ammonia were found to be present in both plasma and erythrocytes, although the deamidation reactions took place solely within the erythrocytes. The total amount of ammonia formed depended on the hemoglobin content of the blood and varied in certain patients suffering from erythrocyte disorders.
对全血静置时氨的自发形成进行了研究,发现这是一系列复杂的反应。氨的形成速率起初很快,但逐渐减慢,大约七天后形成氨的量达到最大。所用抗凝剂的类型以及氧气的可获得性都会影响氨的形成速率。血液冷冻时该反应受到抑制,但发现测定冷冻和解冻血液中的氨含量既困难又不准确。溴化锌稀溶液可部分抑制该反应。虽然脱酰胺反应仅在红细胞内发生,但发现血浆和红细胞中均存在产生氨的化合物。形成的氨总量取决于血液中的血红蛋白含量,并且在某些患有红细胞疾病的患者中有所不同。