TOBE B A
Can Med Assoc J. 1963 Dec 28;89(26):1320-4.
As part of a series of studies into the etiology of acute hepatic encephalopathy the identity of the volatile base, which had been extracted from blood and presumed to be ammonia, was investigated. In order to differentiate between ammonia and the other volatile bases with which it can be confused, a method of separating these compounds by the use of gas chromatography was developed. The technique is described in detail because it incorporates several novel ideas that can be applied to the isolation and identification of similar compounds in many biological systems. The volatile base extracted from blood was found to be ammonia, and no other volatile base was demonstrable in blood from both healthy subjects and patients suffering from acute hepatic encephalopathy.
作为急性肝性脑病病因系列研究的一部分，对从血液中提取的、曾被认为是氨的挥发性碱的成分进行了调查。为了区分氨和可能与之混淆的其他挥发性碱，开发了一种利用气相色谱法分离这些化合物的方法。该技术将作详细描述，因为它包含了几个新颖的想法，可应用于许多生物系统中类似化合物的分离和鉴定。从血液中提取的挥发性碱被发现是氨，在健康受试者和急性肝性脑病患者的血液中均未检测到其他挥发性碱。
