PAGE S G, HUXLEY H E
J Cell Biol. 1963 Nov;19(2):369-90. doi: 10.1083/jcb.19.2.369.
Filament lengths in resting and excited frog muscles have been measured in the electron microscope, and investigations made of the changes in length that are found under different conditions, to distinguish between those changes which arise during preparation and the actual differences in the living muscles. It is concluded that all the measured differences in filament length are caused by the preparative procedures in ways that can be simply accounted for, and that the filament lengths are the same in both resting and excited muscles at all sarcomere lengths greater than 2.1 micro, viz., A filaments, 1.6 micro; I filaments, 2.05 micro. The fine periodicity visible along the I filaments also has been measured in frog, toad, and rabbit muscles and found to be 406 A.
已在电子显微镜下测量了处于静息和兴奋状态的青蛙肌肉中的细丝长度,并对在不同条件下发现的长度变化进行了研究,以区分在制备过程中出现的变化和活体肌肉中的实际差异。得出的结论是,细丝长度的所有测量差异都是由制备程序以简单可解释的方式引起的,并且在所有肌节长度大于2.1微米时,静息和兴奋肌肉中的细丝长度相同,即A细丝为1.6微米;I细丝为2.05微米。还测量了青蛙、蟾蜍和兔子肌肉中沿I细丝可见的精细周期性,发现其为406埃。