MOHOS S C, HENNIGAR G R, FOGELMAN J A
J Exp Med. 1963 Nov 1;118(5):667-80. doi: 10.1084/jem.118.5.667.
An attempt has been made to induce intercapillary glomerulosclerosis in rabbits by immunization with insulin incorporated in Freund's adjuvant and followed by repeated challenges with subcutaneously given insulin. It was observed that lesions resembling human diabetic glomerulosclerosis with occasional nodule-like formation could be produced and that the challenge insulin injections produced proteinuria. The presence of a delayed type of hypersensitivity seemed necessary for the lesions to occur as did the dissemination of the immunizing material to the kidneys. The experiment also disclosed that intravenously given DIS-tagged insulin localizes in a subtly different kind of glomerular lesion with different staining properties. The significance of these findings and the possible role of insulin treatment in the pathogenesis of human diabetic glomerulosclerosis is discussed.
人们尝试通过用弗氏佐剂包裹胰岛素免疫兔子，随后皮下反复注射胰岛素进行激发，来诱导家兔发生毛细血管间肾小球硬化症。观察到可以产生类似于人类糖尿病性肾小球硬化症且偶有结节样形成的病变，并且激发性胰岛素注射会导致蛋白尿。迟发型超敏反应的存在似乎对于病变的发生是必要的，免疫物质向肾脏的扩散也是如此。该实验还揭示，静脉注射带有DIS标签的胰岛素会定位在一种具有不同染色特性的细微不同类型的肾小球病变中。讨论了这些发现的意义以及胰岛素治疗在人类糖尿病性肾小球硬化症发病机制中的可能作用。
