Di Mario U, Ventriglia L, Iavicoli M, Guy K, Andreani D
Clin Exp Immunol. 1983 Jun;52(3):575-80.
The relationship between immune complexes and insulin antibodies was evaluated in 237 insulin treated subjects with a duration of diabetes of more than 1 year. Ninety-seven diabetics were selected at random (group 1) whereas 140 according to the presence of diabetic microangiopathy (group 2). Immune complexes were evaluated by the solid phase C1q binding test in all patients and by conglutinin radioimmune assay in most of them. Insulin antibodies were determined by Christiansen's and Anderson's methods. Immune complexes as detected by the solid phase C1q method were found increased in group 1 and there was an inverse correlation between these complexes and insulin antibody levels. In group 2 patients with microangiopathy the amount of this kind of complex was significantly greater than in those without microvascular lesions and there was no correlation with insulin antibodies. Immune complexes as detected by conglutinin were found increased in group 2 patients and these were significantly correlated with the level of insulin antibodies. No increase in these immune complexes was found in patients with microangiopathy when compared with patients without microangiopathy. Insulin antibodies were not correlated with the presence of complications. Overall, immune complexes detected by C1q binding were significantly correlated with the presence of microangiopathy. In patients with high insulin antibody levels the complexes formed were not detected by C1q binding. The immune complexes detected by conglutinin are correlated with insulin antibodies, but not with the presence of microangiopathy.
对237名糖尿病病程超过1年且接受胰岛素治疗的患者的免疫复合物与胰岛素抗体之间的关系进行了评估。随机选取97名糖尿病患者（第1组），而另外140名则根据是否存在糖尿病微血管病变进行分组（第2组）。所有患者均通过固相C1q结合试验评估免疫复合物，大多数患者还通过胶固素放射免疫测定法进行评估。胰岛素抗体通过克里斯蒂安森法和安德森法测定。通过固相C1q法检测发现第1组的免疫复合物增加，且这些复合物与胰岛素抗体水平呈负相关。在第2组有微血管病变的患者中，这类复合物的量明显高于无微血管病变的患者，且与胰岛素抗体无相关性。通过胶固素检测发现第2组患者的免疫复合物增加，且这些复合物与胰岛素抗体水平显著相关。与无微血管病变的患者相比，有微血管病变的患者中这些免疫复合物没有增加。胰岛素抗体与并发症的存在无关。总体而言，通过C1q结合检测到的免疫复合物与微血管病变的存在显著相关。在胰岛素抗体水平高的患者中，C1q结合未检测到形成的复合物。通过胶固素检测到的免疫复合物与胰岛素抗体相关，但与微血管病变的存在无关。
