SALVIN S B, SMITH R F
J Exp Med. 1964 May 1;119(5):851-68. doi: 10.1084/jem.119.5.851.
Adult guinea pigs were made unresponsive to a heterologous protein (e.g. bovine gamma globulin, or BGG) or a hapten-protein conjugate (e.g. p-aminobenzoic acid-bovine gamma globulin, or PABAgammamiddot;BGG) by intraperitoneal injection of 80 mg cyclophosphamide and the specific antigen. This immunologic unresponsiveness developed to the specific antigen administered simultaneously with the cyclophosphamide, and not to any variants. Thus, animals unresponsive to PABAgammamiddot;BGG remained unresponsive to the original antigen on challenge with a variant, but formed delayed hypersensitivity and circulating antibody to the variant. The specificity of immunologic unresponsiveness, therefore, seems more closely related to the whole antigen molecule than does delayed hypersensitivity.
通过腹腔注射80毫克环磷酰胺和特异性抗原,使成年豚鼠对异种蛋白(如牛γ球蛋白,或BGG)或半抗原 - 蛋白偶联物(如对氨基苯甲酸 - 牛γ球蛋白,或PABAgammamiddot;BGG)无反应。这种免疫无反应性是针对与环磷酰胺同时给予的特异性抗原产生的,而不是针对任何变体。因此,对PABAgammamiddot;BGG无反应的动物在用变体攻击时,对原始抗原仍无反应,但对变体形成迟发型超敏反应和循环抗体。因此,免疫无反应性的特异性似乎比迟发型超敏反应更紧密地与整个抗原分子相关。