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人血清中两种主要遗传性群体特异性成分的分离与部分特性鉴定

ISOLATION AND PARTIAL CHARACTERIZATION OF THE TWO PRINCIPAL INHERITED GROUP-SPECIFIC COMPONENTS OF HUMAN SERUM.

作者信息

CLEVE H, PRUNIER J H, BEARN A G

出版信息

J Exp Med. 1963 Nov 1;118(5):711-26. doi: 10.1084/jem.118.5.711.

DOI:10.1084/jem.118.5.711

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2137678/

Abstract

The group-specific component (Gc), an alpha(2)-globulin of human plasma with inherited variations in relative electrophoretic mobility, has been isolated from plasma and partially characterized. The isolation procedure combines ammonium sulfate fractionation, anion exchange chromatography, preparative zone electrophoresis, and gel filtration. The method is suitable for the isolation and purification of the group-specific components. The Gc proteins representing the gene products of the two common homozygous Gc-types) Gc 1-1 and Gc 2-2, have been prepared. Gc belongs to the group of alpha(2)-globulins of relatively low molecular weight (4.1S, molecular weight 50,800) and relatively low carbohydrate content (3.3 per cent). The total amino acid composition of the two homozygous group-specific components is very similar; treatment with reducing agents and alkylation provides no evidence for the presence of more than a single polypeptide chain in the Gc molecule.

摘要

群体特异性成分（Gc）是人类血浆中的一种α2球蛋白，其相对电泳迁移率存在遗传变异，已从血浆中分离出来并进行了部分特性鉴定。分离过程结合了硫酸铵分级分离、阴离子交换色谱、制备区带电泳和凝胶过滤。该方法适用于群体特异性成分的分离和纯化。已经制备出了代表两种常见纯合Gc型（Gc 1-1和Gc 2-2）基因产物的Gc蛋白。Gc属于相对低分子量（4.1S，分子量50,800）和相对低糖含量（3.3%）的α2球蛋白组。两种纯合群体特异性成分的总氨基酸组成非常相似；用还原剂处理和烷基化未提供证据表明Gc分子中存在不止一条多肽链。