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1
The second component of human complement: its isolation, fragmentation by C'1 esterase, and incorporation into C'3 convertase.人补体的第二个成分：其分离、被C'1酯酶裂解以及并入C'3转化酶。
J Exp Med. 1968 Sep 1;128(3):533-51. doi: 10.1084/jem.128.3.533.
2
Complement as a mediator of inflammation. 3. Purification of the activity with anaphylatoxin properties generated by interaction of the first four components of complement and its identification as a cleavage product of C'3.补体作为炎症介质。3. 具有过敏毒素特性的活性物质的纯化，该活性物质由补体前四种成分相互作用产生，并鉴定为C'3的裂解产物。
J Exp Med. 1967 Dec 1;126(6):1027-48. doi: 10.1084/jem.126.6.1027.
3
Formation and functional significance of a molecular complex derived from the second and the fourth component of human complement.源自人补体第二和第四成分的分子复合物的形成及其功能意义。
J Exp Med. 1967 Feb 1;125(2):359-80. doi: 10.1084/jem.125.2.359.
4
THE ROLE OF THE COMPONENTS OF COMPLEMENT IN SPECIFIC IMMUNE FIXATION.补体成分在特异性免疫固定中的作用。
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5
STUDIES ON IMMUNE HUMAN HEMOLYSIS : II. THE DONATH-LANDSTEINER REACTION AS A MODEL SYSTEM FOR STUDYING THE MECHANISM OF ACTION OF COMPLEMENT AND THE ROLE OF C'1 AND C'1 ESTERASE.免疫性人体溶血的研究：Ⅱ.作为研究补体作用机制和 C'1 和 C'1 酯酶作用的模型系统的唐纳特-兰德施泰纳反应。
J Exp Med. 1961 Jan 1;113(1):193-218. doi: 10.1084/jem.113.1.193.
6
The reaction mechanism of beta-1C-globulin (C'3) in immune hemolysis.β-1C球蛋白（补体3，C'3）在免疫溶血中的反应机制。
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7
[Determination of the total complement activity of serum and of 4 fractions: C'1, C'2, C'3, C'4].[血清总补体活性及4个组分（C'1、C'2、C'3、C'4）的测定]
Rev Fr Etud Clin Biol. 1967 Dec;12(10):1024-8.
8
[Determination of total complementary activity and of its 4 fractions: C'1, C'2, C'3 and C'4 in the serum and urine].[血清和尿液中总补体活性及其4个组分（C'1、C'2、C'3和C'4）的测定]
Ann Biol Clin (Paris). 1969 Jul-Sep;27(7):559-70.
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Complement dependent immune phagocytosis. I. Requirements for C'1, C'4, C'2, C'3.补体依赖性免疫吞噬作用。I. 对C'1、C'4、C'2、C'3的需求
Exp Cell Res. 1968 Jul;51(1):45-67. doi: 10.1016/0014-4827(68)90158-4.
10
FUNCTION OF COMPONENTS OF COMPLEMENT IN IMMUNE HEMOLYSIS.补体成分在免疫溶血中的功能。
J Exp Med. 1942 Jul 1;76(1):93-101. doi: 10.1084/jem.76.1.93.

引用本文的文献

1
Complement Proteins as Soluble Pattern Recognition Receptors for Pathogenic Viruses.补体蛋白作为可溶性模式识别受体对致病病毒的作用。
Viruses. 2021 May 2;13(5):824. doi: 10.3390/v13050824.
2
Mechanism of action of the C4 nephritic factor. Deregulation of the classical pathway of C3 convertase.C4肾炎因子的作用机制。C3转化酶经典途径的失调。
J Exp Med. 1981 Jul 1;154(1):1-12. doi: 10.1084/jem.154.1.1.
3
The purification and properties of the second component of guinea-pig complement.豚鼠补体第二成分的纯化及其性质
Biochem J. 1982 Jul 1;205(1):59-67. doi: 10.1042/bj2050059.
4
The binding of human complement component C4 to antibody-antigen aggregates.人补体成分C4与抗体-抗原聚集体的结合。
Biochem J. 1980 Jul 1;189(1):67-80. doi: 10.1042/bj1890067.
5
The human complement system: assembly of the classical pathway C3 convertase.人类补体系统：经典途径C3转化酶的组装。
Biochem J. 1980 Jul 1;189(1):173-81. doi: 10.1042/bj1890173.
6
Regulation of immune complex-mediated complement activation by autoantibodies (F-42) isolated from sera of patients with systemic lupus erythematosus.从系统性红斑狼疮患者血清中分离出的自身抗体（F-42）对免疫复合物介导的补体激活的调节作用
Clin Exp Immunol. 1983 Sep;53(3):541-6.
7
Human platelet activation by C3a and C3a des-arg.C3a和C3a去精氨酸对人血小板的激活作用
J Exp Med. 1983 Aug 1;158(2):603-15. doi: 10.1084/jem.158.2.603.
8
MHC Class III products: an electron microscopic study of the C3 convertases of human complement.主要组织相容性复合体III类产物：人类补体C3转化酶的电子显微镜研究
J Exp Med. 1984 Jan 1;159(1):324-9. doi: 10.1084/jem.159.1.324.
9
Enzymatic treatment transforms trypomastigotes of Trypanosoma cruzi into activators of alternative complement pathway and potentiates their uptake by macrophages.酶处理可将克氏锥虫的锥鞭毛体转化为替代补体途径的激活剂，并增强巨噬细胞对它们的摄取。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):602-5. doi: 10.1073/pnas.78.1.602.
10
Heterogeneity, polypeptide chain composition and antigenic reactivity of autoantibodies (F-42) that are directed against the classical pathway C3 convertase of complement and isolated from sera of patients with systemic lupus erythematosus.从系统性红斑狼疮患者血清中分离出的、针对补体经典途径C3转化酶的自身抗体（F-42）的异质性、多肽链组成及抗原反应性。
Clin Exp Immunol. 1984 Jun;56(3):614-20.

本文引用的文献

1
ISOLATION OF BETA IF-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT.从人血清中分离β-免疫球蛋白并将其鉴定为补体的第五成分。
J Exp Med. 1965 Aug 1;122(2):277-98. doi: 10.1084/jem.122.2.277.
2
C'1 ESTERASE EFFECT ON ACTIVITY AND PHYSICOCHEMICAL PROPERTIES OF THE FOURTH COMPONENT OF COMPLEMENT.C1酯酶对补体第四成分活性及理化性质的影响
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3
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
4
STUDIES ON HUMAN C'1-ESTERASE. II. FUNCTION OF PURIFIED C'1-ESTERASE IN THE HUMAN COMPLEMENT SYSTEM.人类C1酯酶研究。II. 纯化的C1酯酶在人类补体系统中的功能。
J Immunol. 1964 Mar;92:468-78.
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STUDIES ON HUMAN C'1-ESTERASE. I. PURIFICATION AND ENZYMATIC PROPERTIES.人C1酯酶的研究。I. 纯化及酶学性质
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Protein chromatography on calcium phosphate columns.在磷酸钙柱上进行蛋白质色谱分析。
Arch Biochem Biophys. 1956 Nov;65(1):132-55. doi: 10.1016/0003-9861(56)90183-7.
7
Inhibition of immune hemolysis by diisopropyl fluorophosphate.氟磷酸二异丙酯对免疫溶血的抑制作用。
Biochim Biophys Acta. 1955 Oct;18(2):283-4. doi: 10.1016/0006-3002(55)90069-x.
8
Formation and functional significance of a molecular complex derived from the second and the fourth component of human complement.源自人补体第二和第四成分的分子复合物的形成及其功能意义。
J Exp Med. 1967 Feb 1;125(2):359-80. doi: 10.1084/jem.125.2.359.
9
Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum.豚鼠血清中溶血补体九种成分的分离、纯化及测定方法
Immunochemistry. 1966 Mar;3(2):111-35. doi: 10.1016/0019-2791(66)90292-8.
10
C'2ad, an inactive derivative of C'2 released during decay of EAC'4,2a.C'2ad，一种在EAC'4,2a衰变过程中释放的C'2非活性衍生物。
Immunochemistry. 1966 May;3(3):163-76. doi: 10.1016/0019-2791(66)90182-0.

人补体的第二个成分：其分离、被C'1酯酶裂解以及并入C'3转化酶。

The second component of human complement: its isolation, fragmentation by C'1 esterase, and incorporation into C'3 convertase.

作者信息

Polley M J, Müller-Eberhard H J

出版信息

J Exp Med. 1968 Sep 1;128(3):533-51. doi: 10.1084/jem.128.3.533.

DOI:10.1084/jem.128.3.533
PMID:5666963
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2138531/
Abstract

A method has been described for the purification and isolation of the second component of complement (C'2) from human serum. The protein is a beta(1)-globulin with an approximate molecular weight of 117,000. Immunochemical analysis using a variety of specific antisera, including a monospecific antiserum to the isolated protein, indicate that the C'2 protein represents a heretofore unrecognized human serum constituent. Isolated C'2 contained 2 x 10(9) "effective molecules" per microgram and 1000 hemolytically active C'2 molecules were required to produce a single hemolytically effective C'2 site on erythrocytes undergoing immune cytolysis. C'1 esterase treatment of C'2 resulted in reduction of both its electrophoretic mobility and its molecular size, the latter observation indicating fragmentation of the molecule. Direct evidence was presented for the physical presence of C'2 as an integral part of the enzyme C'3 convertase.

摘要

已描述了一种从人血清中纯化和分离补体第二成分（C'2）的方法。该蛋白质是一种β(1)-球蛋白，分子量约为117,000。使用多种特异性抗血清进行的免疫化学分析，包括针对分离出的蛋白质的单特异性抗血清，表明C'2蛋白代表一种迄今未被识别的人血清成分。分离出的C'2每微克含有2×10⁹个“有效分子”，并且在经历免疫细胞溶解的红细胞上产生单个溶血有效C'2位点需要1000个具有溶血活性的C'2分子。用C'1酯酶处理C'2会导致其电泳迁移率和分子大小降低，后一观察结果表明该分子发生了片段化。有直接证据表明C'2作为酶C'3转化酶的一个组成部分实际存在。