Jones P, Watson A, Davies M, Stubbings S
MRC Laboratory of Molecular Biology, MRC Centre, Cambridge, UK.
Nucleic Acids Res. 1992 Sep 11;20(17):4599-606. doi: 10.1093/nar/20.17.4599.
We describe here the integration of image analysis and robotics to produce a fully automated colony picking/plate handling system. Biological tests were performed to verify its performance in terms of sterilisation and accuracy of picking. The machine was then used by a single operative to pick a 36,000 clone cDNA library in approximately 42 hrs over 5 days.
我们在此描述图像分析与机器人技术的整合,以打造一个全自动的菌落挑选/平板处理系统。进行了生物学测试,以验证其在灭菌和挑选准确性方面的性能。然后,一名操作人员使用该机器在5天内约42小时内挑选了一个包含36,000个克隆的cDNA文库。