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将图像分析和机器人技术集成到一个全自动的菌落挑选和平板处理系统中。

Integration of image analysis and robotics into a fully automated colony picking and plate handling system.

作者信息

Jones P, Watson A, Davies M, Stubbings S

机构信息

MRC Laboratory of Molecular Biology, MRC Centre, Cambridge, UK.

出版信息

Nucleic Acids Res. 1992 Sep 11;20(17):4599-606. doi: 10.1093/nar/20.17.4599.

DOI:10.1093/nar/20.17.4599
PMID:1408762
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334190/
Abstract

We describe here the integration of image analysis and robotics to produce a fully automated colony picking/plate handling system. Biological tests were performed to verify its performance in terms of sterilisation and accuracy of picking. The machine was then used by a single operative to pick a 36,000 clone cDNA library in approximately 42 hrs over 5 days.

摘要

我们在此描述图像分析与机器人技术的整合,以打造一个全自动的菌落挑选/平板处理系统。进行了生物学测试,以验证其在灭菌和挑选准确性方面的性能。然后,一名操作人员使用该机器在5天内约42小时内挑选了一个包含36,000个克隆的cDNA文库。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3095/334190/765fbf3506f9/nar00228-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3095/334190/d2a6a3559d86/nar00228-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3095/334190/1cc4b12dca52/nar00228-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3095/334190/765fbf3506f9/nar00228-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3095/334190/d2a6a3559d86/nar00228-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3095/334190/1cc4b12dca52/nar00228-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3095/334190/765fbf3506f9/nar00228-0203-a.jpg

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引用本文的文献

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Getting the Right Clones in an Automated Manner: An Alternative to Sophisticated Colony-Picking Robotics.以自动化方式获得正确的克隆:复杂菌落挑选机器人技术的替代方法。
Bioengineering (Basel). 2024 Sep 3;11(9):892. doi: 10.3390/bioengineering11090892.
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Collaborative Robot Precision Task in Medical Microbiology Laboratory.协作机器人在医学微生物实验室中的精密任务。
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Efficient division and sampling of cell colonies using microcup arrays.利用微孔阵列进行细胞集落的高效分割和取样。

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