Szot P, Myers K M, Dorsa D M
Department of Medicine, University of Washington, Seattle 98195.
Peptides. 1992 Mar-Apr;13(2):389-94. doi: 10.1016/0196-9781(92)90126-n.
Arginine8-vasopressin (AVP, 40 micrograms/100 g b.wt., SC) was administered to male Long-Evans (LE) pups from day 1 to 7 of life and the pups were sacrificed on day 8 or 60. 3H-AVP binding was performed on membranes prepared from the liver, kidney, and septum. No significant changes were observed in the kidney or septum of animals 8 or 60 days old. However, the chronic AVP treatment did result in a significant increase in the density of 3H-AVP binding sites in the liver when compared to control day 8 pups (control 44 +/- 2 vs. AVP 56 +/- 3 fmol/mg protein), with no change in affinity. This effect was maintained into adulthood, as the day 60 AVP-treated LE rats also showed a significant increase in liver 3H-AVP binding sites compared to control (control 186 +/- 9 vs. AVP 239 +/- 14 fmol/mg protein), with no change in affinity. A comparison of 3H-AVP binding sites in 8-day-old LE, heterozygous Brattleboro (HET-BB), and homozygous Brattleboro rats (HOM-BB) was performed to assess the effect of complete (HOM-BB) and partial (HET-BB) VP deficiency on binding sites in the CNS and periphery. The liver again was the only tissue in which a change in 3H-AVP binding characteristics was noted. The HOM-BB rat (Bmax 144 +/- 6 fmol/mg protein) displayed a significant increase in AVP binding sites from the LE rat (Bmax 100 +/- 7 fmol/mg protein), while the 3H-AVP binding sites in the HET-BB rat liver (Bmax 69.8 +/- 9 fmol/mg protein) were significantly lower than LE rats. Thus hepatic AVP receptors appear most sensitive to the presence or absence of vasopressin during the early postnatal period.
从出生第1天至第7天，给雄性Long-Evans（LE）幼崽皮下注射精氨酸8-加压素（AVP，40微克/100克体重），并在第8天或第60天处死幼崽。对从肝脏、肾脏和隔膜制备的膜进行3H-AVP结合实验。在8日龄或60日龄动物的肾脏或隔膜中未观察到显著变化。然而，与对照第8天幼崽相比，慢性AVP处理确实导致肝脏中3H-AVP结合位点的密度显著增加（对照44±2 vs. AVP 56±3飞摩尔/毫克蛋白质），亲和力无变化。这种效应持续到成年期，因为与对照相比，第60天经AVP处理过的LE大鼠肝脏中的3H-AVP结合位点也显著增加（对照186±9 vs. AVP 239±14飞摩尔/毫克蛋白质），亲和力无变化。对8日龄LE、杂合Brattleboro（HET-BB）和纯合Brattleboro大鼠（HOM-BB）的3H-AVP结合位点进行比较，以评估完全（HOM-BB）和部分（HET-BB）VP缺乏对中枢神经系统和外周结合位点的影响。肝脏再次是唯一观察到3H-AVP结合特征发生变化的组织。纯合Brattleboro大鼠（Bmax 144±6飞摩尔/毫克蛋白质）的AVP结合位点比LE大鼠（Bmax为100±7飞摩尔/毫克蛋白质）显著增加，而杂合Brattleboro大鼠肝脏中的3H-AVP结合位点（Bmax 69.8±9飞摩尔/毫克蛋白质）显著低于LE大鼠。因此，肝脏AVP受体在出生后早期似乎对加压素的存在或缺失最为敏感。
