脑线粒体。一、牛脑线粒体的分离
BRAIN MITOCHONDRIA. I. ISOLATION OF BOVINE BRAIN MITOCHONDRIA.
作者信息
STAHL W L, SMITH J C, NAPOLITANO L M, BASFORD R E
出版信息
J Cell Biol. 1963 Nov;19(2):293-307. doi: 10.1083/jcb.19.2.293.
Abstract
Two methods of preparing a mitochondrial fraction from beef brain cortex are described. Data are presented on the rate of oxidation of substrates, P/O and respiratory control ratios, cholinesterase activity, and DNA content. Electron micrographs of isolated mitochondria and mitochondria in situ are shown. Comparisons are drawn between these preparations and mitochondria prepared in 0.25 M sucrose. The data on enzymic properties and contamination by non-mitochondrial material indicate that mitochondrial fractions which compare favorably with those from other tissues can be prepared from brain tissue.
摘要
本文描述了两种从牛脑皮质制备线粒体组分的方法。文中给出了底物氧化速率、P/O和呼吸控制率、胆碱酯酶活性以及DNA含量的数据。展示了分离线粒体和原位线粒体的电子显微照片。对这些制备物与在0.25M蔗糖中制备的线粒体进行了比较。关于酶学性质和非线粒体物质污染的数据表明，可以从脑组织中制备出与其他组织的线粒体组分相媲美的线粒体组分。
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