Gottstein B, Deplazes P, Aubert M
Institute of Parasitology, University of Zürich, Switzerland.
Parasitol Res. 1992;78(4):291-7. doi: 10.1007/BF00937086.
Echinococcus multilocularis oncospheres, primary vesicular cysts, and protoscolices were assessed in vitro and in vivo for their potential to synthesize a PAS-positive laminated layer containing monoclonal antibody (mAb) G11-binding Em2 antigen. The presence of Em2 antigen in developed oncospheres and cysts was subsequently correlated to the potential of in vivo development into a secondary metacestode in recipient host mice, which also responded by anti-Em2 serum antibody formation. In contrast, protoscolices failed to develop the "Em2-positive" layer in vitro under the selected experimental conditions. The failure to develop subsequently in vivo into a secondary metacestode was underlined by a lack of anti-Em2 serum antibody formation by the hosts. We furthermore developed a technique to obtain E. multilocularis clones by inoculating single oncospheres into recipient mice.
在体外和体内评估了多房棘球绦虫六钩蚴、初级囊状囊肿和原头节合成含单克隆抗体（mAb）G11结合Em2抗原的PAS阳性分层膜的潜力。随后，发育的六钩蚴和囊肿中Em2抗原的存在与在受体宿主小鼠体内发育成继发性绦虫蚴的潜力相关，受体宿主小鼠也通过抗Em2血清抗体形成做出反应。相比之下，在选定的实验条件下，原头节在体外未能形成“Em2阳性”层。宿主缺乏抗Em2血清抗体形成，突出表明其随后在体内未能发育成继发性绦虫蚴。此外，我们还开发了一种通过将单个六钩蚴接种到受体小鼠中来获得多房棘球绦虫克隆的技术。
