Bi Xiao-Juan, Shao Yin-Mei, Li Liang, Wang Yan, Zhang Chuan-Shan, Lü Guo-Dong, Aji Tuerganaili, Li Jun, Zhang Wen-Bao, Wen Hao, Lin Ren-Yong
State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, P.R. China.
Hepatobiliary & Hydatid Department, Digestive and Vascular Surgery Centre, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, P.R. China.
Exp Ther Med. 2018 Oct;16(4):3155-3160. doi: 10.3892/etm.2018.6555. Epub 2018 Aug 1.
Alveolar echinococcosis (AE) is a prevalent epidemic in the northern hemisphere, especially in central Europe and western China. Serum diagnosis is important for patients with AE, especially during the first screening. The present study purified the recombinant Em18-GST (rEm18-GST), and detected its diagnostic performance in human alveolar echinococcosis patients of Xinjiang, China with immunoblotting (IB) and enzyme-linked immunosorbent assay (ELISA). Serum samples were collected from 50 patients with AE, 222 patients with cystic echinococcosis (CE), 158 patients with other unrelated infections and 106 healthy individuals. The IB results showed that serum samples of 47 patients with AE and 12 patients with CE were rEm18-positive. However, only one sample from patients with cancer showed a cross-reaction with rEm18 in IB. The overall sensitivity was 94%, and the total specificity was 96.58%. For the rEm18 results using ELISA, the sera of 46 patients with AE were positive, and the overall sensitivity was 92%. In conclusion, compared with imaging tools, including computed tomography, magnetic resonance imaging and positron emission tomography, rEm18 has considerable advantages for AE serodiagnosis.
肺泡型棘球蚴病（AE）在北半球流行，尤其是在中欧和中国西部。血清学诊断对AE患者很重要，尤其是在初次筛查时。本研究纯化了重组Em18-GST（rEm18-GST），并通过免疫印迹法（IB）和酶联免疫吸附测定法（ELISA）检测其在中国新疆肺泡型棘球蚴病患者中的诊断性能。收集了50例AE患者、222例囊性棘球蚴病（CE）患者、158例其他无关感染患者和106例健康个体的血清样本。IB结果显示，47例AE患者和12例CE患者的血清样本rEm18呈阳性。然而，仅1例癌症患者的样本在IB中与rEm18出现交叉反应。总体敏感性为94%，总特异性为96.58%。对于ELISA检测的rEm18结果，46例AE患者的血清呈阳性，总体敏感性为92%。总之，与包括计算机断层扫描、磁共振成像和正电子发射断层扫描在内的成像工具相比，rEm18在AE血清学诊断方面具有相当大的优势。
