Smart D, Johnston C F, Curry W J, Shaw C, Halton D W, Fairweather I, Buchanan K D
Comparative Neuroendocrinology Research Group, School of Clinical Medicine, Queen's University, Belfast, Northern Ireland, UK.
Parasitol Res. 1992;78(4):329-35. doi: 10.1007/BF00937092.
Antisera to a highly conserved region of chromogranin A (sequence KELTAE) and to a hexapeptide (sequence KGQELE) adjacent to the putative C-terminus of pancreastatin, a peptide whose sequence is found within the chromogranin A molecule, have been used to examine the localisation of immunoreactivity (IR) to these peptides in Ascaris suum. IR to both peptides was found in the nerve rings and nerve cords. In addition, KGQELE-IR was also observed in the pharyngeal neurones and in a network of fibres on the surface of the female gonoduct. The staining was specific in that it could be abolished by preincubation of the antisera with the appropriate antigen. The two antisera appeared to be staining different subsets of neurones, suggesting that (at least) two peptides were being recognised. The wide-spread distribution of IR to both peptides throughout the nervous system of the parasite suggests that the peptides carrying the epitopes recognised by the antisera are of fundamental importance to the functioning of the parasite's nervous system.
针对嗜铬粒蛋白A高度保守区域（序列KELTAE）以及与胰抑制素假定C末端相邻的六肽（序列KGQELE）的抗血清已被用于检测猪蛔虫中这些肽的免疫反应性（IR）定位。在猪蛔虫中，神经环和神经索中均发现了对这两种肽的IR。此外，在咽部神经元以及雌性生殖管道表面的纤维网络中也观察到了KGQELE-IR。这种染色具有特异性，因为用适当抗原预孵育抗血清可消除染色。这两种抗血清似乎在对不同的神经元亚群进行染色，表明（至少）识别出了两种肽。在寄生虫神经系统中广泛分布的对这两种肽的IR表明，携带抗血清所识别表位的肽对寄生虫神经系统的功能至关重要。
