Brownlee D J, Fairweather I, Johnston C F, Smart D, Shaw C, Halton D W
Comparative Neuroendocrinology Research Group, School of Biology and Biochemistry, Queen's University of Belfast, Northern Ireland.
Parasitology. 1993 Apr;106 ( Pt 3):305-16. doi: 10.1017/s0031182000075132.
The localization and distribution of neuropeptides in the central nervous system of the pig roundworm, Ascaris suum, have been determined by an indirect immunofluorescence technique in conjunction with confocal microscopy. Antisera to 25 vertebrate peptides and two invertebrate peptides were used to screen the worm for immunoreactivity (IR). Immunostaining was obtained with antisera to pancreatic polypeptide (PP), peptide YY (PYY), neuropeptide Y (NPY), gastrin, cholecystokinin (CCK), substance P (SP), atrial natriuretic peptide (ANP), salmon gonadotropin-releasing hormone (SGnRH), mammalian gonadotropin-releasing hormone (MGnRH), chromogranin A (CGA) and FMRFamide. The most extensive patterns of IR occurred with antisera to PYY, FMRFamide and gastrin. IR was evident in nerve cells and fibres in the ganglia associated with the anterior nerve ring and in the main nerve cords and their commissures; IR to FMRFamide also occurred in the posterior nerve ring. Immunostaining for the other peptides was confined to the nerve cords, with the number of immunoreactive nerve fibres varying from peptide to peptide.
利用间接免疫荧光技术结合共聚焦显微镜，已确定了猪蛔虫（猪巨吻棘头虫）中枢神经系统中神经肽的定位和分布。使用针对25种脊椎动物肽和两种无脊椎动物肽的抗血清来筛选该蠕虫的免疫反应性（IR）。用针对胰多肽（PP）、肽YY（PYY）、神经肽Y（NPY）、胃泌素、胆囊收缩素（CCK）、P物质（SP）、心钠素（ANP）、鲑鱼促性腺激素释放激素（SGnRH）、哺乳动物促性腺激素释放激素（MGnRH）、嗜铬粒蛋白A（CGA）和FMRF酰胺的抗血清获得了免疫染色。IR最广泛的模式出现在针对PYY、FMRF酰胺和胃泌素的抗血清中。IR在前神经环相关神经节的神经细胞和纤维以及主神经索及其连合中很明显；对FMRF酰胺的IR也出现在后神经环中。其他肽的免疫染色局限于神经索，免疫反应性神经纤维的数量因肽而异。
