转化的中国仓鼠卵巢细胞中荧光素酶分泌的成像。

Inouye S, Ohmiya Y, Toya Y, Tsuji F I

Osaka Bioscience Institute, Japan.

Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9584-7. doi: 10.1073/pnas.89.20.9584.

The blue luminescence characteristic of the marine ostracod crustacean Vargula hilgendorfii is from a simple, but highly specific, enzyme-substrate reaction. Light is emitted by the oxidation of Vargula luciferin (substrate) by molecular oxygen, a reaction catalyzed by luciferase. Stable transformants of Chinese hamster ovary cells carrying the Vargula luciferase gene secreted luciferase from discrete sites on the cell surface, and this secretion could be monitored in real time by the bioluminescence produced by the secreted luciferase in the presence of Vargula luciferin by using an image-intensifying technique. Addition of anti-Vargula luciferase IgG to the luminescing cells almost completely extinguished the luminescence, confirming that Vargula luciferase caused the luminescence.

海洋介形纲甲壳动物希氏亮糠虾的蓝色发光特性源自一种简单但高度特异的酶-底物反应。光由分子氧氧化亮糠虾荧光素（底物）产生，该反应由荧光素酶催化。携带亮糠虾荧光素酶基因的中国仓鼠卵巢细胞稳定转化体从细胞表面的离散位点分泌荧光素酶，利用图像增强技术，通过分泌的荧光素酶在亮糠虾荧光素存在时产生的生物发光可实时监测这种分泌。向发光细胞中添加抗亮糠虾荧光素酶IgG几乎完全消除了发光，证实亮糠虾荧光素酶导致了发光。