Department of Physical Therapy and Rehabilitation Science, College of Health Science, Eulji University, Gyeonggi 13135, Korea.
Sensors (Basel). 2017 Jun 6;17(6):1306. doi: 10.3390/s17061306.
Since articular cartilage has a limited regeneration potential, for developing biological therapies for cartilage regeneration it is important to study the mechanisms underlying chondrogenesis of stem cells. Bioluminescence assays can visualize a wide range of biological phenomena such as gene expression, signaling, metabolism, development, cellular movements, and molecular interactions by using visible light and thus contribute substantially to elucidation of their biological functions. This article gives a concise review to introduce basic principles of bioluminescence assays and applications of the technology to visualize the processes of chondrogenesis and cartilage regeneration. Applications of bioluminescence assays have been highlighted in the methods of real-time monitoring of gene expression and intracellular levels of biomolecules and noninvasive cell tracking within animal models. This review suggests that bioluminescence assays can be applied towards a visual understanding of chondrogenesis and cartilage regeneration.
由于关节软骨的再生潜力有限，因此开发用于软骨再生的生物疗法，研究干细胞成软骨的机制非常重要。生物发光检测法可以通过可见光来可视化广泛的生物现象，如基因表达、信号转导、代谢、发育、细胞运动和分子相互作用，从而极大地促进对其生物学功能的阐明。本文简要综述了生物发光检测法的基本原理及其在可视化成软骨和软骨再生过程中的应用。生物发光检测法在实时监测基因表达和细胞内生物分子水平以及在动物模型中进行非侵入性细胞追踪的方法中的应用已得到强调。本文综述表明，生物发光检测法可用于直观地理解成软骨和软骨再生。
