肝脏中叶酸活性的测定
THE ASSAY OF FOLIC-ACID ACTIVITY OF LIVER.
作者信息
BENNETT M C, BERRY V, CHANARIN I, ARDEMAN S
出版信息
J Clin Pathol. 1964 Jan;17(1):27-30. doi: 10.1136/jcp.17.1.27.
Abstract
Methods of preparing liver for the microbiological assay of folic-acid activity have been compared. Maximum values were obtained by incubating an alkaline homogenate of liver in the presence of ascorbic acid for five hours at 37 degrees C. Most other procedures resulted in significant loss of folic-acid activity.
摘要
已对用于叶酸活性微生物学测定的肝脏制备方法进行了比较。通过在37摄氏度下于抗坏血酸存在的情况下将肝脏的碱性匀浆孵育5小时可获得最大值。大多数其他方法导致叶酸活性显著损失。
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本文引用的文献
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The nature of the glutamic acid excreted in folic acid deficiency.叶酸缺乏时所排泄的谷氨酸的性质。
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The folic acid activity of human livers as measured with Lactobacillus casei.用人型干酪乳杆菌测定的人肝脏的叶酸活性。
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Availability of citrovorum factor in natural materials.天然物质中醛氢叶酸的可利用性。
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