Aboko-Cole G F, Lee C M
Z Parasitenkd. 1975;45(3):281-90. doi: 10.1007/BF00329380.
An experimental model composed of the folic acid deficient Sprague-Dawley rat and Trypanosoma rhodesiense was used to study folate levels in sera and tissues. Serum folate levels in well fed rats inoculated on day 21 averaged 21 plus or minus ng/ml; well fed normal rats averaged 18 plus or minus 4 ng/ml. In rats given the pair-fed control diet, serum folate levels averaged 17.2 plus or minus 4 ng/ml for trypanosome-free animals and 20.2 plus or minus 3 ng/ml for infected ones. In rats given the folic acid-deficient diet, serum folate levels averaged 8.6 plus or minus 2 ng/ml for noninfected control animals and 9.3 plus or minus 2 ng/ml for trypanosome-infected ones. Regardless of diet, the infected animals inoculated on day 56 had higher serum folate levels over the controls on the last day of observation (day 5 of infection). Livers from rats fed complete and pair-fed diets and inoculated on day 21 showed no significant differences in folate content when compared to control animals. However, livers of rats on a deficient diet showed significantly more folate when compared with uninfected controls, reaching a maximum of 362% of day 25. Liver folate levels of rats (regardless of the dietary regimen) inoculated on day 56 showed significantly higher values than the controls on day 60. Irrespective of the time of inoculation or diet, brain and spinal cord of T. rhodesiense-infected rats had significantly higher folate values than their controls on day 5 of infection. The folate level of the brain and spinal cord, at this time, ranged up to 58 and 107% respectively.
使用由叶酸缺乏的斯普拉格-道利大鼠和罗德西亚锥虫组成的实验模型来研究血清和组织中的叶酸水平。在第21天接种的营养良好的大鼠血清叶酸水平平均为21±ng/ml；营养良好的正常大鼠平均为18±4 ng/ml。在给予配对喂养对照饮食的大鼠中，未感染锥虫的动物血清叶酸水平平均为17.2±4 ng/ml，感染锥虫的动物为20.2±3 ng/ml。在给予叶酸缺乏饮食的大鼠中，未感染的对照动物血清叶酸水平平均为8.6±2 ng/ml，感染锥虫的动物为9.3±2 ng/ml。无论饮食如何，在第56天接种的感染动物在观察的最后一天（感染第5天）的血清叶酸水平高于对照组。在第21天接种的喂食完全饮食和配对喂养饮食的大鼠肝脏与对照动物相比，叶酸含量没有显著差异。然而，与未感染对照相比，缺乏饮食的大鼠肝脏显示出显著更多的叶酸，在第25天达到最高362%。在第56天接种的大鼠（无论饮食方案如何）肝脏叶酸水平在第60天显示出显著高于对照组的值。无论接种时间或饮食如何，在感染第5天，感染罗德西亚锥虫的大鼠的脑和脊髓叶酸值显著高于其对照组。此时，脑和脊髓的叶酸水平分别高达58%和107%。
