BOOTHROYD B
J Cell Biol. 1964 Jan;20(1):165-73. doi: 10.1083/jcb.20.1.165.
Thin sections of embryonic avian bone decalcify during preparation for electron microscopy, creating a false impression of mineral distribution. The results of the experiments reported herein show that viscous embedding materials do not penetrate compact formed bone, and so, in thin sections, the calcium apatite crystals may be leeched out by water, both in the collecting trough and in aqueous solutions of stains used to enhance tissue electron opacity. To prevent decalcification, a simple technique is described in which the aqueous fluids that come in contact with thin sections are saturated with respect to calcium and phosphate ions, thereby preventing solution of the bone mineral. The theoretical basis of this technique is briefly discussed.
在制备用于电子显微镜观察的胚胎禽骨薄片时,骨会脱钙,从而产生矿物质分布的假象。本文报道的实验结果表明,粘性包埋材料无法穿透致密的成熟骨,因此,在薄片中,钙磷灰石晶体可能会在收集槽以及用于增强组织电子不透明度的染色水溶液中被水浸出。为防止脱钙,本文描述了一种简单技术,即让与薄片接触的水性液体中钙和磷酸根离子达到饱和,从而防止骨矿物质溶解。本文还简要讨论了该技术的理论基础。