SEED J
J Cell Biol. 1964 Jan;20(1):17-23. doi: 10.1083/jcb.20.1.17.
Interferometric and photometric measurements have been made successively on individual cell nuclei derived from normal embryonic tissues and spontaneous tumour tissues of the mouse grown in vivo. From the measurements, the relations between nucleic acid and dry mass content have been studied in the two types of nuclei and the results shown to be consistent with differences in cell metabolism previously reported to exist in vitro. In the nuclei of normal embryonic cells, the syntheses of DNA, nuclear RNA, and protein appear to be closely associated, whereas in the tumour cell nuclei an appreciable fraction of the chromatin RNA and protein synthesis is dissociated from the replication of DNA.
已对源自体内生长的小鼠正常胚胎组织和自发肿瘤组织的单个细胞核相继进行了干涉测量和光度测量。通过这些测量,研究了两种类型细胞核中核酸与干物质含量之间的关系,结果表明与先前报道的体外存在的细胞代谢差异一致。在正常胚胎细胞的细胞核中,DNA、核RNA和蛋白质的合成似乎紧密相关,而在肿瘤细胞核中,相当一部分染色质RNA和蛋白质合成与DNA复制解离。