DUTTON R W, EADY J D
Immunology. 1964 Jan;7(1):40-53.
The uptake of [C]thymidine by spleen cell suspensions from rabbits previously immunized to heterologous serum proteins is stimulated by the addition of antigen . This stimulation is specific and dependent upon concentration of antigen. It represents an increased rate of DNA synthesis and cell division by certain cells of the suspension. These cells are large undifferentiated mononuclear cells comprising approximately 1 per cent of the total population. It is suggested that this response is the counterpart of the cellular proliferation seen in whole animals following the re-injection of antigen. The procedure described provides a quantitative system for the study of the mechanism of antigenic stimulation. Some preliminary experiments are reported.
给先前用异种血清蛋白免疫过的家兔的脾细胞悬液添加抗原后,可刺激其对[C]胸苷的摄取。这种刺激具有特异性,且依赖于抗原浓度。它代表着悬液中某些细胞的DNA合成速率和细胞分裂速率增加。这些细胞是未分化的大单核细胞,约占细胞总数的1%。有人认为,这种反应相当于再次注射抗原后在完整动物体内所见到的细胞增殖。所描述的方法为研究抗原刺激机制提供了一个定量系统。本文报道了一些初步实验。