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Antigen-specific proliferation responses of peripheral blood lymphocytes to Trichomonas vaginalis antigen in patients with Trichomonas vaginitis.滴虫性阴道炎患者外周血淋巴细胞对阴道毛滴虫抗原的抗原特异性增殖反应。
J Clin Microbiol. 1983 Feb;17(2):175-80. doi: 10.1128/jcm.17.2.175-180.1983.
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[Macrophage activation and lymphokine production in the mouse by lymphocytes sensitized against Trichomonas vaginalis].[阴道毛滴虫致敏淋巴细胞对小鼠巨噬细胞的激活及淋巴因子的产生]
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Treatment of infections caused by metronidazole-resistant Trichomonas vaginalis.耐甲硝唑阴道毛滴虫引起的感染的治疗。
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本文引用的文献

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AN IN VITRO SYSTEM FOR THE STUDY OF THE MECHANISM OF ANTIGENIC STIMULATION IN THE SECONDARY RESPONSE.用于研究二次反应中抗原刺激机制的体外系统。
Immunology. 1964 Jan;7(1):40-53.
2
On the venereal nature of genito-urinary trichomoniasis.关于泌尿生殖系统滴虫病的性病性质。
J Hyg Epidemiol Microbiol Immunol. 1963;7:240-51.
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Lymphocyte transformation in the diagnosis of congenital toxoplasma infection.淋巴细胞转化在先天性弓形虫感染诊断中的应用
N Engl J Med. 1980 Apr 3;302(14):785-8. doi: 10.1056/NEJM198004033021406.
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Immune response to Trichomonas vaginalis. I. Genetic regulation of IgE antibody response to T. vaginalis in mice.对阴道毛滴虫的免疫反应。I. 小鼠对阴道毛滴虫IgE抗体反应的遗传调控。
Int Arch Allergy Appl Immunol. 1982;67(4):310-4.
5
Delayed hypersensitivity in the guinea-pig to a protein-hapten conjugate and its relationship to in vitro transformation of lymph node, spleen, thymus and peripheral blood lymphocytes.豚鼠对蛋白质-半抗原结合物的迟发型超敏反应及其与淋巴结、脾脏、胸腺和外周血淋巴细胞体外转化的关系。
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Genetically determined immune responses: in vitro studies.基因决定的免疫反应：体外研究
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Pleural fluid and peripheral blood lymphocyte function in tuberculosis.结核病中胸腔积液与外周血淋巴细胞功能
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8
T lymphocyte-enriched murine peritoneal exudate cells. I. A reliable assay for antigen-induced T lymphocyte proliferation.富含T淋巴细胞的小鼠腹腔渗出细胞。I. 一种用于抗原诱导T淋巴细胞增殖的可靠检测方法。
J Immunol. 1975 Nov;115(5):1330-8.

滴虫性阴道炎患者外周血淋巴细胞对阴道毛滴虫抗原的抗原特异性增殖反应。

Antigen-specific proliferation responses of peripheral blood lymphocytes to Trichomonas vaginalis antigen in patients with Trichomonas vaginitis.

作者信息

Yano A, Aosai F, Yui K, Kojima S, Kawana T

出版信息

J Clin Microbiol. 1983 Feb;17(2):175-80. doi: 10.1128/jcm.17.2.175-180.1983.

DOI:10.1128/jcm.17.2.175-180.1983
PMID:6601112
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272604/
Abstract

This report describes the development of an assay system which overcomes the difficulty of detecting immune responses of patients with Trichomonas vaginitis by making use of peripheral blood leukocytes obtained from such patients. When peripheral blood leukocytes from the patient were stimulated in microcultures with the soluble antigen extracted from Trichomonas vaginalis, significant degrees of proliferation ensued, as measured by the incorporation of [methyl-3H]thymidine 4 to 5 days after initiation. The antigen-induced proliferation response of peripheral blood leukocytes is specific for T. vaginalis antigen. The T. vaginalis-specific [methyl-3H]thymidine incorporation is mediated by Leu-1-positive cells, namely, T lymphocytes, in the peripheral blood leukocyte population. This assay system should prove useful for the analysis of the immune response to the protozoa in patients with Trichomonas vaginitis.

摘要

本报告描述了一种检测系统的开发，该系统通过利用从滴虫性阴道炎患者获取的外周血白细胞，克服了检测此类患者免疫反应的困难。当用从阴道毛滴虫中提取的可溶性抗原在微量培养中刺激患者的外周血白细胞时，在培养开始后4至5天通过[甲基-3H]胸腺嘧啶核苷掺入量测定，会出现显著程度的增殖。外周血白细胞的抗原诱导增殖反应对阴道毛滴虫抗原具有特异性。阴道毛滴虫特异性的[甲基-3H]胸腺嘧啶核苷掺入是由外周血白细胞群体中Leu-1阳性细胞，即T淋巴细胞介导的。该检测系统应证明对分析滴虫性阴道炎患者对该原生动物的免疫反应有用。