硫氧还蛋白还原酶缺陷型大肠杆菌突变体的分离
Isolation of an Escherichia coli mutant deficient in thioredoxin reductase.
作者信息
Fuchs J
出版信息
J Bacteriol. 1977 Feb;129(2):967-72. doi: 10.1128/jb.129.2.967-972.1977.
Abstract
A mutant of Escherichia coli defective in thioredoxin reductase has been isolated and partially characterized. This mutant has no detectable thioredoxin reductase activity in vitro and yet it exhibits no in vivo defect in reduction of ribonucleotides. Evidence is presented that indicates that, in cells permeabilized via ether treatment, ribonucleoside diphosphate reduction can utilize glutathione as an alternate reducing system.
摘要
已分离出一种硫氧还蛋白还原酶有缺陷的大肠杆菌突变体，并对其进行了部分特性分析。该突变体在体外没有可检测到的硫氧还蛋白还原酶活性，但在核糖核苷酸还原方面没有体内缺陷。有证据表明，在经乙醚处理而透性化的细胞中，核糖核苷二磷酸还原可利用谷胱甘肽作为替代还原系统。
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